Targeted Δ16bp null mutation in the first translated exon of Zfp800, generated via CRISPR/Cas9 endonuclease technology. C57Bl6J, Cas9 expressing mice were crossed to C57Bl6N wildtype mice to generate Cas9+ embryos. These embryos were injected with target specific gRNAs and the resulting founders are in a mixed C57Bl6J/ C57Bl6N background. Donor males were 75% C57Bl6/J and 25% C57Bl6/N at cryopreservation.


Record History
Added on April 20, 2021 at 2:45 PM by Skelton, Jennifer
Modified on April 20, 2021 at 3:03 PM by Skelton, Jennifer
Shared with (contributions)
VCMR: Vanderbilt Cryopreserved Mouse Repository Master
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Mouse Information

Common Name Zfp800
VCMR ID YY
Date Cryopreserved 2018-06-26
Method of Cryopreservation Sperm
Trial IVF % Fertilization 25.00%

Genetic Alteration

Mutation #1: Nuclease-Mediated
Allele Name: zinc finger protein 800; endonuclease mediated 1, Mark Magnuson
Symbol: Zfp800em1Mgn
Zygosity at cryopreservation Heterozygous
PCR Genotyping Protocol Genotyping_Protocol_YY.pdf
Citations
Publication
A developmental lineage-based gene co-expression network for mouse pancreatic β-cells reveals a role for in pancreas development. (2021) Development 148: (Added 3/15/2021)
PMID: 33653874

Background Strain Information

Strain Type Inbred Strain
Chimera/Founder Genetic Background C57BL/6NHsd
Cryopreservation Strain Background (VCMR) C57BL/6J
Viability and Fertility Data

Viable and fertile.