Knockout of a gene that encodes Nrf2 which encodes exons 4 and 5.  It mirrors the genetic change found in the Nfe2l2 null mouse.  The loxP/FRT flanked Neo cassette is inserted on the 3' side of exon 5 and the single loxP site is inserted on the 5' side of exon 4.  The target region is 2843 bps and includes exons 4-5.  Chimeric mice were crossed to FLP mice to remove the Neo cassette. 


Record History
Added on April 12, 2021 at 1:12 PM by Skelton, Jennifer
Modified on April 12, 2021 at 1:20 PM by Skelton, Jennifer
Shared with (contributions)
VCMR: Vanderbilt Cryopreserved Mouse Repository Master
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Mouse Information

Common Name Nfe2l2 Knockout
VCMR ID PB
Date Cryopreserved 2013-04-09
Method of Cryopreservation Sperm
Trial IVF % Fertilization 66.00%

Genetic Alteration

Mutation #1: Targeted Mutagenesis
Allele Name: nuclear factor, erythroid derived 2, like 2, targeted mutation 1.1
Symbol: Nfe2l2tm1.1
Zygosity at cryopreservation Homozygous
PCR Genotyping Protocol NRFT_EP82_Feb_2010.doc
Citations
Publication
Loss of Nrf2 promotes alveolar type 2 cell loss in irradiated, fibrotic lung. (2017) Free Radic Biol Med 112: 578-586 (Added 4/12/2021)
PMID: 28870520

Background Strain Information

Strain Type Congenic Strain
Chimera/Founder Genetic Background 129
Cryopreservation Strain Background (VCMR) C57BL/6J
Viability and Fertility Data

Viable and fertile

Attachments

Document NRFT_crelox_TV_DOC.doc - Added on April 12, 2021 at 1:11 PM by Jennifer Skelton

NRFT: Construction of Conditional Targeting Vector


Document Supplemental_Information__revised_1_Submitted_8_28... - Added on April 12, 2021 at 1:11 PM by Jennifer Skelton

PMID:28870520 Supplemental Information