A C57Bl6/NHsd strain contains a knockout allele of Slc3a1. This animal was created to study cystinuria, a human disease involving kidney stones.


Record History
Added on December 18, 2020 at 10:08 AM by Skelton, Jennifer
Modified on December 18, 2020 at 10:18 AM by Skelton, Jennifer
Shared with (contributions)
VCMR: Vanderbilt Cryopreserved Mouse Repository Master
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Mouse Information

Common Name Slc3a1/Tyr -/-
VCMR ID ABV
Date Cryopreserved 2020-12-08
Method of Cryopreservation Sperm
Trial IVF % Fertilization 81.97%

Genetic Alteration

Mutation #1: Nuclease-Mediated
Allele Name: solute carrier family 3, member 1
Symbol: Slc3a1em1
Zygosity at cryopreservation Homozygous
PCR Genotyping Protocol Wilson_Slc3A1_Genotyping_Protocol.pdf
Citations
Publication
CRISPR/Cas9 engineering of albino cystinuria Type A mice. (2020) Genesis 58: e23357 (Added 3/3/2020)
PMID: 32078250

Background Strain Information

Strain Type Congenic Strain
Chimera/Founder Genetic Background C57BL/6NHsd
Cryopreservation Strain Background (VCMR) C57BL/6NHsd
Viability and Fertility Data

These mice are viable as both heterozygotes and homozygotes. Litter sizes can be small (2-3) to moderate (7-9) in size with larger litter sizes very uncommon. Fertility has been noted to be difficult at times usually during lower humidity winter months, but tends to rebound during more humid months.