Fam120b-/- mice were backcrossed for 2 generations to Sox2-Cre C57Bl6 mice.  The FLP mice used to remove the lacZ site was Gt(ROSA)26Sor<tm1(FLP1)Dym.  Global knockout was achieved using Sox2-Cre mice, Edil3Tg(Sox2-Cre)1AMC.


Record History
Added on August 17, 2020 at 11:36 AM by Skelton, Jennifer
Modified on August 17, 2020 at 12:33 PM by Skelton, Jennifer
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VCMR: Vanderbilt Cryopreserved Mouse Repository Master
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Mouse Information

Common Name San1-/-;Fam120B-/-
VCMR ID YT
Date Cryopreserved 2018-06-12
Method of Cryopreservation Sperm
Trial IVF % Fertilization 14.00%

Genetic Alterations

Mutation #1: BAC or Transgene Insertion
Expressed Gene Name: EGF-like repeats and discoidin I-like domains 3
Symbol: Edil3
NCBI Gene ID: 13612
MGI: 1329025
Zygosity at cryopreservation Homozygous
PCR Genotyping Protocol SAN1KOmice1.pdf
Citations Not provided
Mutation #2: Targeted Mutagenesis
Allele Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Susan Dymecki
Symbol: Gt(ROSA)26Sortm1(FLP1)Dym
MGI: MGI:2429412
Zygosity at cryopreservation Homozygous
PCR Genotyping Protocol Not provided
Citations Not provided
Mutation #3: Targeted Mutagenesis
Allele Name: family with sequence similarity 120, member B; targeted mutation 1a, Hemholtz Zentrum Muenchen GmbH
Symbol: Fam120btm1a(EUCOMM)Hmgu
MGI: MGI:4458741
Zygosity at cryopreservation Homozygous
PCR Genotyping Protocol SAN1KOmice1.pdf
Citations
Publication
A senataxin-associated exonuclease SAN1 is required for resistance to DNA interstrand cross-links. (2018) Nat Commun 9: 2592 (Added 8/17/2020)
PMID: 29968717

Background Strain Information

Strain Type Mixed
Chimera/Founder Genetic Background C57BL/6NHsd
Cryopreservation Strain Background (VCMR) C57BL/6NHsd
Viability and Fertility Data

Viable and fertile; no overt phenotype