A neo/ura3 fragment flanked by LoxP sites was used to replace the first three Myo1a exons via yeast-mediated homologous recombination. Absence of protein in mutants was confirmed by immunoblot of small intestines. Immunostaining of mutant duodenal sections with an antibody directed against the TH1 domain showed the expected lack of signal in mutants.


Record History
Added on August 4, 2020 at 1:29 PM by Skelton, Jennifer
Modified on August 4, 2020 at 1:34 PM by Skelton, Jennifer
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VCMR: Vanderbilt Cryopreserved Mouse Repository Master
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Mouse Information

Common Name Myosin1A KO
VCMR ID OU
Date Cryopreserved 2013-01-11
Method of Cryopreservation Sperm
Trial IVF % Fertilization 86.00%

Genetic Alteration

Mutation #1: Targeted Mutagenesis
Allele Name: myosin IA; targeted mutation 1, Matthew J Tyska
Symbol: Myo1atm1Mtys
MGI: 3587749
Zygosity at cryopreservation Homozygous
PCR Genotyping Protocol Myo1A_Genotyping.docx
Citations
Publication
Myosin-1a is critical for normal brush border structure and composition. (2005) Mol Biol Cell 16: 2443-57 (Added 5/19/2014)
PMID: 15758024

Background Strain Information

Strain Type Mixed
Chimera/Founder Genetic Background 129S1/SvImJ
Cryopreservation Strain Background (VCMR) 129S1/SvImJ
Viability and Fertility Data Not provided