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B10.129S6-Tsc2tm1.1Kcess/Vu
Floxed mutant mice possessing flanking loxP sites of the Tsc2 gene on a C57Bl/10J background.
- Record History
- Added on May 19, 2020 at 10:58 AM by Skelton, Jennifer
Modified on May 19, 2020 at 11:06 AM by Skelton, Jennifer - Shared with (contributions)
- VCMR: Vanderbilt Cryopreserved Mouse Repository
Mouse Information
| Common Name | Tsc2 Flox |
|---|---|
| VCMR ID | RQ |
| Date Cryopreserved | 2014-01-02 |
| Method of Cryopreservation | Sperm |
| Trial IVF % Fertilization | 32.00% |
Genetic Alteration
| Mutation #1: Targeted Mutagenesis | |||
|---|---|---|---|
| Allele |
Name: TSC complex subunit 2; targeted mutation 1.1, Kevin C Ess Symbol: Tsc2tm1.1Kcess MGI: 5496783 |
||
| Zygosity at cryopreservation | Homozygous | ||
| PCR Genotyping Protocol | Tsc2_flox_genotyping_primers.docx | ||
| Citations |
|
||
Background Strain Information
| Strain Type | Congenic Strain |
|---|---|
| Chimera/Founder Genetic Background | 129S6/SvEvTac |
| Cryopreservation Strain Background (VCMR) | C57Bl/10J |
| Viability and Fertility Data | Homozygous and heterozygous viable and fertile. |
Additional Information
A loxP site was inserted upstream of exon 36. An FRT-flanked neomycin resistance cassette with a 3' loxP site was inserted downstream of exon 37. Flp-mediated recombination removed the neomycin resistance cassette and left exons 36 and 37 floxed.
