Floxed mutant mice possessing flanking loxP sites of the Tsc2 gene on a C57Bl/10J background.

Record History
Added on May 19, 2020 at 10:58 AM by Skelton, Jennifer
Modified on May 19, 2020 at 11:06 AM by Skelton, Jennifer
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VCMR: Vanderbilt Cryopreserved Mouse Repository Master
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Mouse Information

Common Name Tsc2 Flox
Date Cryopreserved 2014-01-02
Method of Cryopreservation Sperm
Trial IVF % Fertilization 32.00%

Genetic Alteration

Mutation #1: Targeted Mutagenesis
Allele Name: TSC complex subunit 2; targeted mutation 1.1, Kevin C Ess
Symbol: Tsc2tm1.1Kcess
MGI: 5496783
Zygosity at cryopreservation Homozygous
PCR Genotyping Protocol Tsc2_flox_genotyping_primers.docx
Conditional and domain-specific inactivation of the Tsc2 gene in neural progenitor cells. (2013) Genesis 51: 284-92 (Added 12/2/2013)
PMID: 23359422

Background Strain Information

Strain Type Congenic Strain
Chimera/Founder Genetic Background 129S6/SvEvTac
Cryopreservation Strain Background (VCMR) C57Bl/10J
Viability and Fertility Data

Homozygous and heterozygous viable and fertile.

Additional Information

A loxP site was inserted upstream of exon 36.  An FRT-flanked neomycin resistance cassette with a 3' loxP site was inserted downstream of exon 37.  Flp-mediated recombination removed the neomycin resistance cassette and left exons 36 and 37 floxed.