Common Name | Tsc2 Flox |
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VCMR ID | RQ |
Date Cryopreserved | 2014-01-02 |
Method of Cryopreservation | Sperm |
Trial IVF % Fertilization | 32.00% |
Mutation #1: Targeted Mutagenesis | |||
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Allele |
Name: TSC complex subunit 2; targeted mutation 1.1, Kevin C Ess Symbol: Tsc2tm1.1Kcess MGI: 5496783 |
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Zygosity at cryopreservation | Homozygous | ||
PCR Genotyping Protocol | Tsc2_flox_genotyping_primers.docx | ||
Citations |
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Strain Type | Congenic Strain |
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Chimera/Founder Genetic Background | 129S6/SvEvTac |
Cryopreservation Strain Background (VCMR) | C57Bl/10J |
Viability and Fertility Data | Homozygous and heterozygous viable and fertile. |
A loxP site was inserted upstream of exon 36. An FRT-flanked neomycin resistance cassette with a 3' loxP site was inserted downstream of exon 37. Flp-mediated recombination removed the neomycin resistance cassette and left exons 36 and 37 floxed.