A -7bp deletion in the exon 1 of Zfp92 was generated using CRISPR/Cas. C57Bl6/J-Cas9 expressing mice were crossed to C57Bl6/N wildtype mice to generate Cas9+ embryos.  These embryos were injected with target-specific gRNAs.  Donor males were 93.75% C57Bl6/J and 6.25% C57Bl6/N at cryopreservation.


Record History
Added on August 30, 2019 at 1:15 PM by Skelton, Jennifer
Modified on March 15, 2021 at 4:15 PM by Skelton, Jennifer
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VCMR: Vanderbilt Cryopreserved Mouse Repository Master
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Mouse Information

Common Name Zfp92
VCMR ID ZN
Date Cryopreserved 2019-04-30
Method of Cryopreservation Sperm
Trial IVF % Fertilization 63.00%

Genetic Alteration

Mutation #1: Nuclease-Mediated
Allele Name: zinc finger protein 92; endonuclease mediated 1, Mark Magnuson
Symbol: Zfp92em1Mgn
Zygosity at cryopreservation Homozygote
PCR Genotyping Protocol Zfp92em1Mgn_Genotyping_Protocol.docx
Citations Not provided

Background Strain Information

Strain Type Inbred Strain
Chimera/Founder Genetic Background C57Bl6/J x C57Bl6/N
Cryopreservation Strain Background (VCMR) C57BL/6
Viability and Fertility Data

Viable and fertile

93.27% C57Bl6/J at cryopreservation.

Backcrossed 1 generation onto C57Bl6/J at cryopreservation.

Additional Information

The gene is on the X chromosome, males will have one knockout allele on the X chromosome and the other allele will be absent due to the Y chromosome.

Attachment

Document Magnuson_Neogen_Analysis_Zfp92.pdf - Added on December 20, 2019 at 2:55 PM by Jennifer Skelton