A -8bp deletion in exon 1 of Jazf1 was generated using CRISPR/Cas9.  Cas9+ embryos were obtained by crossing C57Bl6/J-Cas9 expressing mice with C57Bl6/N mice and injected with target-specific gRNAs.  Donor males were 75% C57Bl6/J and 25% C57Bl6/N at cryopreservation.


Record History
Added on August 30, 2019 at 1:05 PM by Skelton, Jennifer
Modified on March 15, 2021 at 4:14 PM by Skelton, Jennifer
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VCMR: Vanderbilt Cryopreserved Mouse Repository Master
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Mouse Information

Common Name Jazf1
VCMR ID ZO
Date Cryopreserved 2019-04-30
Method of Cryopreservation Sperm
Trial IVF % Fertilization 71.00%

Genetic Alteration

Mutation #1: Nuclease-Mediated
Allele Name: JAZF zinc finger 1; endonuclease mediated 1, Mark Magnuson
Symbol: Jazf1em1Mgn
Zygosity at cryopreservation Heterozygote
PCR Genotyping Protocol Jazf1em1Mgn_Genotyping_Protocol.docx
Citations Not provided

Background Strain Information

Strain Type Inbred Strain
Chimera/Founder Genetic Background C57Bl6/J x C57Bl6/N
Cryopreservation Strain Background (VCMR) C57BL/6
Viability and Fertility Data

Viable and fertile

75% C57Bl6/J and 25% C57Bl6/N at cryopreservation.

Backcrossed 1 generation onto C57Bl6/J at cryopreservation.

Attachment

Document Magnuson_Neogen_Analysis_JazF1.pdf - Added on December 20, 2019 at 2:54 PM by Jennifer Skelton