An 8 bp, frame-shifting deletion in exon 1 of Zfp329 was generated using CRISPR/Cas. Cas9+ embryos were obtained by crossing C57Bl6/J-Cas9 expressing mice with C57Bl6/N mice and injected with target-specific gRNAs. Donor males were 87.5% C57Bl6/J and 12.5% C57Bl6/N at cryopreservation.

Record History
Added on August 30, 2019 at 11:55 AM by Skelton, Jennifer
Modified on August 30, 2019 at 3:21 PM by Magnuson, Mark
Shared with (contributions)
VCMR: Vanderbilt Cryopreserved Mouse Repository Master

Mouse Information

Common Name Zfp329
Research Applications Not provided
MMRRC ID Not provided
Jackson Laboratories Stock No Not provided
Additional Strain Information Not provided

Genetic Alteration

Mutation #1: Nuclease-Mediated
Allele Name: zinc finger protein 329; endonuclease mediated 1; Mark Magnuson
Symbol: Zfp329em1Mgn
PCR Genotyping Protocol Zfp329_Genotyping_Protocol.docx
Citations Not provided

Background Strain Information

Strain Type Inbred Strain
Chimera/Founder Genetic Background C57Bl6/J x C57Bl6/N
Current Genetic Background C57BL/6
Number of Generations Backcrossed 2
Strain Description

C57Bl6/J, Cas9 expressing mice were crossed to C57Bl6/N wildtype mice to generate Cas9+ embryos.  These embryos were injected with target-specific gRNAs and the resulting founders are in a mixed C57Bl6J/C57Bl6N background.  The cryopreserved strain contains a mix of C57Bl6/J and C57Bl6/N strains and is ~87% C57Bl6/J.