An 8 bp, frame-shifting deletion in exon 1 of Zfp329 was generated using CRISPR/Cas. Cas9+ embryos were obtained by crossing C57Bl6/J-Cas9 expressing mice with C57Bl6/N mice and injected with target-specific gRNAs. Donor males were 87.5% C57Bl6/J and 12.5% C57Bl6/N at cryopreservation.


Record History
Added on August 30, 2019 at 11:55 AM by Skelton, Jennifer
Modified on August 30, 2019 at 3:21 PM by Magnuson, Mark
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VCMR: Vanderbilt Cryopreserved Mouse Repository Master
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Mouse Information

Common Name Zfp329
VCMR ID ZQ
Date Cryopreserved 2019-05-07
Method of Cryopreservation Sperm
Trial IVF % Fertilization 97.00%

Genetic Alteration

Mutation #1: Nuclease-Mediated
Allele Name: zinc finger protein 329; endonuclease mediated 1; Mark Magnuson
Symbol: Zfp329em1Mgn
Zygosity at cryopreservation Heterozygote
PCR Genotyping Protocol Zfp329_Genotyping_Protocol.docx
Citations Not provided

Background Strain Information

Strain Type Inbred Strain
Chimera/Founder Genetic Background C57Bl6/J x C57Bl6/N
Cryopreservation Strain Background (VCMR) C57BL/6
Viability and Fertility Data

Viable and fertile.

87.5% C57Bl6/J and 12.5% C57Bl6/N at cryopreservation.

Backcrossed 2 generations at cryopreservation onto C57Bl6/J.