A 7 bp, frame-shifting deletion in exon 1 of the Zfhx4 gene was generated using CRISPR/Cas9.  Cas9+ embryos were obtained by crossing C57Bl6/J-Cas9 expressing mice with C57Bl6/N mice and injected with target-specific gRNAs. Donor males were 75% C57Bl6/J and 25% C57Bl6/N at cryopreservation.


Record History
Added on August 28, 2019 at 3:29 PM by Skelton, Jennifer
Modified on August 30, 2019 at 3:18 PM by Magnuson, Mark
Shared with (contributions)
VCMR: Vanderbilt Cryopreserved Mouse Repository Master

Mouse Information

Common Name Zfhx4
Research Applications Not provided
MMRRC ID Not provided
Jackson Laboratories Stock No Not provided
VCMR ID ZP
Additional Strain Information Not provided

Genetic Alteration

Mutation #1: Nuclease-Mediated
Allele Name: zinc finger homeodomain 4; endonuclease mediated 1, Mark Magnuson
Symbol: Zfhx4em1Mgn
PCR Genotyping Protocol Zfhx4_Genotyping_Protocol.docx
Citations Not provided

Background Strain Information

Strain Type Inbred Strain
Chimera/Founder Genetic Background C57Bl6/J x C57Bl6/N
Current Genetic Background C57BL/6
Number of Generations Backcrossed 1
Strain Description Not provided

Attachment

Document Zfhx4_gentotyping_8.1.18.pptx - Added on August 30, 2019 at 1:24 PM by Jennifer Skelton