A 7 bp, frame-shifting deletion in exon 1 of the Zfhx4 gene was generated using CRISPR/Cas9.  Cas9+ embryos were obtained by crossing C57Bl6/J-Cas9 expressing mice with C57Bl6/N mice and injected with target-specific gRNAs. Donor males were 75% C57Bl6/J and 25% C57Bl6/N at cryopreservation.


Record History
Added on August 28, 2019 at 3:29 PM by Skelton, Jennifer
Modified on August 30, 2019 at 3:18 PM by Magnuson, Mark
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VCMR: Vanderbilt Cryopreserved Mouse Repository Master
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Mouse Information

Common Name Zfhx4
VCMR ID ZP
Date Cryopreserved 2019-05-07
Method of Cryopreservation Sperm
Trial IVF % Fertilization 70.00%

Genetic Alteration

Mutation #1: Nuclease-Mediated
Allele Name: zinc finger homeodomain 4; endonuclease mediated 1, Mark Magnuson
Symbol: Zfhx4em1Mgn
Zygosity at cryopreservation Heterozygote
PCR Genotyping Protocol Zfhx4_Genotyping_Protocol.docx
Citations Not provided

Background Strain Information

Strain Type Inbred Strain
Chimera/Founder Genetic Background C57Bl6/J x C57Bl6/N
Cryopreservation Strain Background (VCMR) C57BL/6
Viability and Fertility Data

Viable and fertile

75% C57Bl6/J and 25% C57Bl6/N at cryopreservation.  

Backcrossed one time onto C57Bl6/J background at cryopreservation.

 

Attachment

Document Zfhx4_gentotyping_8.1.18.pptx - Added on August 30, 2019 at 1:24 PM by Jennifer Skelton