Common Name | Gcg-RSR-CreERT2 |
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VCMR ID | ZZ |
Date Cryopreserved | 2019-08-13 |
Method of Cryopreservation | Sperm |
Trial IVF % Fertilization | 31.00% |
Mutation #1: Targeted Mutagenesis | |||
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Allele |
Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 14, Hongkui Zeng Symbol: Gt(ROSA)26Sortm14(CAG-tdTomato)Hze MGI: 3809524 |
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Zygosity at cryopreservation | Heterozygous | ||
PCR Genotyping Protocol | Not provided | ||
Citations |
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Mutation #2: Targeted Mutagenesis | |||
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Allele |
Name: targeted mutation 1 Symbol: GcgRSR-CreERT2 |
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Zygosity at cryopreservation | Heterozygous | ||
PCR Genotyping Protocol | GcgRSRCreERT2_Genotyping_protocol.docx | ||
Citations |
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Strain Type | Congenic Strain |
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Chimera/Founder Genetic Background | 129S6.SvEvTac x C57Bl/6NCr F1 derived G4 ES cells |
Cryopreservation Strain Background (VCMR) | C57BL/6J |
Viability and Fertility Data | While heterozygous mice have no phenotype, homozygous mice are deficient for Gcg gene-derived peptides and are not fertile. Backcrossed 5 generations onto C57Bl6/J at cryopreservation. |
Sperm was pooled from a GcgRSR-CreERT2/w male and a GcgRSR-CreERT2/w; Rosa26tdTomoato/w male.
GcgRSRCreERT2_allele_map.docx - Added on August 13, 2019 at 9:57 AM by Jennifer Skelton
Shiota_Neogen_Analysis_GcgRSRCreERT2.pdf - Added on December 20, 2019 at 2:50 PM by Jennifer Skelton