The Dre-dependent glucagon-CreERT2 knock-in/knock-out strain was designed to express CreERT2 only in pancreatic alpha-cells among cells expressing glucagon gene, i.e. pancreatic alpha-cells, Gcg neurons, and intestinal L cells, using a Cre/Dre dual recombination system. Gcg-RSR-CreERT2 mice have the same modification of glucagon (Gcg) gene as Gcg-CreERT2 mice (Pubmed ID: 28884202) except for a rox-flanked STOP cassette (RSR) inserted upstream of CreERT2. Expression of CreERT2 driven by the endogenous Gcg gene promoter occurs only after the removal of RSR by Dre recombinase. When bred to Pdx1-Dre mice (PMID: 31160417), the resulting offspring with both Gcg_RSR-CreERT2 and Pdx1-Dre genes will have CreERT2 expression in pancreatic alpha-cells, but not in Gcg neurons.

Record History
Added on August 13, 2019 at 9:58 AM by Skelton, Jennifer
Modified on September 8, 2020 at 3:55 PM by Magnuson, Mark
Shared with (contributions)
VCMR: Vanderbilt Cryopreserved Mouse Repository Master
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Mouse Information

Common Name Gcg-RSR-CreERT2
Date Cryopreserved 2019-08-13
Method of Cryopreservation Sperm
Trial IVF % Fertilization 31.00%

Genetic Alterations

Mutation #1: Targeted Mutagenesis
Allele Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 14, Hongkui Zeng
Symbol: Gt(ROSA)26Sortm14(CAG-tdTomato)Hze
MGI: 3809524
Zygosity at cryopreservation Heterozygous
PCR Genotyping Protocol Not provided
A robust and high-throughput Cre reporting and characterization system for the whole mouse brain. (2010) Nat Neurosci 13: 133-40 (Added 8/13/2019)
PMID: 20023653
Mutation #2: Targeted Mutagenesis
Allele Name: targeted mutation 1
Symbol: GcgRSR-CreERT2
Zygosity at cryopreservation Heterozygous
PCR Genotyping Protocol GcgRSRCreERT2_Genotyping_protocol.docx
Gcg knockin mice as a tool for genetic manipulation in pancreatic alpha cells. (2017) Diabetologia 60: 2399-2408 (Added 8/13/2019)
PMID: 28884202

Background Strain Information

Strain Type Congenic Strain
Chimera/Founder Genetic Background 129S6.SvEvTac x C57Bl/6NCr F1 derived G4 ES cells
Cryopreservation Strain Background (VCMR) C57BL/6J
Viability and Fertility Data

While heterozygous mice have no phenotype, homozygous mice are deficient for Gcg gene-derived peptides and are not fertile.

Backcrossed 5 generations onto C57Bl6/J at cryopreservation.

Additional Information

Sperm was pooled from a GcgRSR-CreERT2/w male and a GcgRSR-CreERT2/w; Rosa26tdTomoato/w male.



Document GcgRSRCreERT2_allele_map.docx - Added on August 13, 2019 at 9:57 AM by Jennifer Skelton

Document Shiota_Neogen_Analysis_GcgRSRCreERT2.pdf - Added on December 20, 2019 at 2:50 PM by Jennifer Skelton