- Allele validation in the N1
generation. CRISPR mutations in the F0 generation can
often be a problem to sort out. Thus, for new gene editing projects
we now offer the option of having the VGER breed and genotype N1
generation offspring for you. This allows a repeat validation of
the induced mutation in a heterozygous animal.
Analysis: CRISPR has the potential to generate off
target mutations. If this is a concern for you, we can design and
perform PCR assays to detect editing at predicted off-target
- Random Insertion
Analysis: DNA donor sequences, particularly longer
ones, can insert randomly in the genome. We can design and perform
assays to detect random insertions and use this information to help
you decide which founders to breed.
- Rapid Colony Expansion and
Cryopreservation. It can be very time consuming to
establish a colony of experimental animals from a single founder.
If time is critical for you, we can harvest sperm from N1
heterozygous mice and use it to fertilize multiple isogenic wild
type embryos. Aggressive breeding of the resulting heterozygous
mice can accelerate experimental analysis by saving two or more
generations of natural mating.
To initiate a genome editing project, contact Leesa Sampson at