Prostaglandin E receptor 2 (subtype EP2) targeted null/knockout on 129S6 background.  

Mice that are homozygous for the targeted mutation are viable, normal in size and do not display any gross physical or behavioral abnormalities. Reduced fertility in homozygous females is due to a pre-implantation defect. Fewer eggs are released during ovulation, of which fewer are fertilized and implanted when compared to wildtype controls, resulting in smaller litters (3 pups/litter). Mutant mice display slightly elevated baseline systolic blood pressure. Prostaglandin 2 infusion or high salt diet causes systolic hypertension in homozygous mice. This mutant mouse strain represents a model that may be useful in studies of salt sensitive hypertension and infertility. Strain of origin is 129S6/SvEvTac.

Record History
Added on June 18, 2019 at 2:21 PM by Skelton, Jennifer
Modified on September 5, 2019 at 1:40 PM by Skelton, Jennifer
Shared with (contributions)
VCMR: Vanderbilt Cryopreserved Mouse Repository Master
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Mouse Information

Common Name EP2null129
Date Cryopreserved 2009-09-09
Method of Cryopreservation Sperm
Trial IVF % Fertilization 88.00%

Genetic Alteration

Mutation #1: Targeted Mutagenesis
Allele Name: prostaglandin E receptor 2 (subtype EP2); targeted mutation 1, Richard M Breyer
Symbol: Ptger2tm1Brey
MGI: 2179967
Zygosity at cryopreservation Homozygous
PCR Genotyping Protocol EP2null_PCR_protocol.pdf
Salt-sensitive hypertension and reduced fertility in mice lacking the prostaglandin EP2 receptor. (1999) Nat Med 5: 217-20 (Added 12/21/2013)
PMID: 9930871

Background Strain Information

Strain Type Congenic Strain
Chimera/Founder Genetic Background 129S6/SvEvTac
Cryopreservation Strain Background (VCMR) 129S6/SvEvTac
Viability and Fertility Data

No known viability or fertility abnormalities.

Strain Background:  Backcrossed >10 generations onto 129S6 at cryopreservation.


Document EP2null_PCR_protocol.pdf - Added on June 18, 2019 at 2:21 PM by Jennifer Skelton