Prostaglandin E receptor 2 (subtype EP2) targeted null/knockout on C57Bl/6 background. 

Mice that are homozygous for the targeted mutation are viable, normal in size and do not display any gross physical or behavioral abnormalities. Reduced fertility in homozygous females is due to a pre-implantation defect. Fewer eggs are released during ovulation, of which fewer are fertilized and implanted when compared to wildtype controls, resulting in smaller litters (3 pups/litter). Mutant mice display slightly elevated baseline systolic blood pressure. Prostaglandin 2 infusion or high salt diet causes systolic hypertension in homozygous mice. This mutant mouse strain represents a model that may be useful in studies of salt sensitive hypertension and infertility. Strain of origin is 129S6/SvEvTac backcrossed onto C57Bl/6 more than 10 times. 


Record History
Added on June 18, 2019 at 2:03 PM by Skelton, Jennifer
Modified on June 18, 2019 at 2:06 PM by Skelton, Jennifer
Shared with (contributions)
VCMR: Vanderbilt Cryopreserved Mouse Repository Master

Mouse Information

Common Name EP2nullB6
Research Applications Not provided
MMRRC ID Not provided
Jackson Laboratories Stock No Not provided
VCMR ID ER
Additional Strain Information Not provided

Genetic Alteration

Mutation #1: Targeted Mutagenesis
Type of AlleleConditional Null
Targeted GeneName: prostaglandin E receptor 2 (subtype EP2)
Symbol: Ptger2
NCBI: 19217
Allele Name: targeted mutation 1
Symbol: Ptger2tm1Brey
MGI: 3179967
Description of Targeting Vector Not provided
Vector Genbank File Not provided
Allele Map Not Provided
PCR Genotyping Protocol EP2null_PCR_protocol.pdf
Citations
Publication
Salt-sensitive hypertension and reduced fertility in mice lacking the prostaglandin EP2 receptor. (1999) Nat Med 5: 217-20 (Added 12/21/2013)
PMID: 9930871

Background Strain Information

Strain Type Congenic Strain
Chimera/Founder Genetic Background 129S6/SvEvTac
Current Genetic Background C57BL/6J
Number of Generations Backcrossed >10
Strain Description Not provided

Attachments

Document EP2nullB6_supporting_info.docx - Added on June 18, 2019 at 2:03 PM by Jennifer Skelton


Document EP2null_PCR_protocol.pdf - Added on June 18, 2019 at 2:03 PM by Jennifer Skelton