Prostaglandin E receptor 2 (subtype EP2) targeted null/knockout on C57Bl/6 background. 

Mice that are homozygous for the targeted mutation are viable, normal in size and do not display any gross physical or behavioral abnormalities. Reduced fertility in homozygous females is due to a pre-implantation defect. Fewer eggs are released during ovulation, of which fewer are fertilized and implanted when compared to wildtype controls, resulting in smaller litters (3 pups/litter). Mutant mice display slightly elevated baseline systolic blood pressure. Prostaglandin 2 infusion or high salt diet causes systolic hypertension in homozygous mice. This mutant mouse strain represents a model that may be useful in studies of salt sensitive hypertension and infertility. Strain of origin is 129S6/SvEvTac backcrossed onto C57Bl/6 more than 10 times. 


Record History
Added on June 18, 2019 at 2:03 PM by Skelton, Jennifer
Modified on June 18, 2019 at 2:06 PM by Skelton, Jennifer
Shared with (contributions)
VCMR: Vanderbilt Cryopreserved Mouse Repository Master
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Mouse Information

Common Name EP2nullB6
VCMR ID ER
Date Cryopreserved 2005-05-23
Method of Cryopreservation Embryo

Genetic Alteration

Mutation #1: Targeted Mutagenesis
Allele Name: targeted mutation 1
Symbol: Ptger2tm1Brey
MGI: 3179967
Zygosity at cryopreservation Homozygous
PCR Genotyping Protocol EP2null_PCR_protocol.pdf
Citations
Publication
Salt-sensitive hypertension and reduced fertility in mice lacking the prostaglandin EP2 receptor. (1999) Nat Med 5: 217-20 (Added 12/21/2013)
PMID: 9930871

Background Strain Information

Strain Type Congenic Strain
Chimera/Founder Genetic Background 129S6/SvEvTac
Cryopreservation Strain Background (VCMR) C57BL/6J
Viability and Fertility Data

Infertility in homozygous null females, use heterozygous females for breeding.

Attachments

Document EP2nullB6_supporting_info.docx - Added on June 18, 2019 at 2:03 PM by Jennifer Skelton


Document EP2null_PCR_protocol.pdf - Added on June 18, 2019 at 2:03 PM by Jennifer Skelton