Prostaglandin E receptor 2 (subtype EP2) targeted null/knockout on Balb/c background.

Mice that are homozygous for the targeted mutation are viable, normal in size and do not display any gross physical or behavioral abnormalities. Reduced fertility in homozygous females is due to a pre-implantation defect. Fewer eggs are released during ovulation, of which fewer are fertilized and implanted when compared to wildtype controls, resulting in smaller litters (3 pups/litter). Mutant mice display slightly elevated baseline systolic blood pressure. Prostaglandin 2 infusion or high salt diet causes systolic hypertension in homozygous mice. This mutant mouse strain represents a model that may be useful in studies of salt sensitive hypertension and infertility. Strain of origin is 129S6/SvEvTac backcrossed onto Balb/c more than 10 times. 


Record History
Added on June 18, 2019 at 1:54 PM by Skelton, Jennifer
Modified on September 5, 2019 at 1:40 PM by Skelton, Jennifer
Shared with (contributions)
VCMR: Vanderbilt Cryopreserved Mouse Repository Master
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Mouse Information

Common Name EP2nullBAL
VCMR ID EY
Date Cryopreserved 2005-09-26
Method of Cryopreservation Embryo

Genetic Alteration

Mutation #1: Targeted Mutagenesis
Allele Name: prostaglandin E receptor 2 (subtype EP2); targeted mutation 1, Richard M Breyer
Symbol: Ptger2tm1Brey
MGI: 3179967
Zygosity at cryopreservation Homozygous
PCR Genotyping Protocol EP2null_PCR_protocol.pdf
Citations
Publication
Prostaglandin E2 receptor subtype 2 (EP2) null mice are protected against murine lung tumorigenesis. (2006) Anticancer Res 26: 2857-61 (Added 12/21/2013)
PMID: 16886605

Background Strain Information

Strain Type Congenic Strain
Chimera/Founder Genetic Background 129S6/SvEvTac
Cryopreservation Strain Background (VCMR) BALB/c
Viability and Fertility Data

No known issues with viability or fertility.

Attachments

Document EP2nullBAL_supporting_info.docx - Added on June 18, 2019 at 1:54 PM by Jennifer Skelton


Document EP2null_PCR_protocol.pdf - Added on June 18, 2019 at 1:54 PM by Jennifer Skelton