Floxed mutant mice possessing loxP sites which flank exon 2 of the Ptger4 gene.  

Prostaglandin E2 (PGE2), a fatty acid derivative and a primary target of NSAIDs, is important in diverse physiological processes, including but not limited to: contraction and relaxation of smooth muscle, vasodilation, vasoconstriction, blood pressure regulation, immune response and inflammation regulation and bone formation and healing. There are four receptors for PGE2. The Ptger4 gene encodes a G-protein coupled PGE2 receptor (EP4), which is essential for embryonic development and neonatal survival in mice. 
These mice possess loxP sites on either side of exon 2 of the targeted gene. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissues. Removal of the floxed sequence creates a null allele. During backcrossing, the Y chromosome may not have been fixed to the C57BL/6J genetic background.


Record History
Added on June 18, 2019 at 1:29 PM by Skelton, Jennifer
Modified on August 9, 2019 at 11:46 AM by Skelton, Jennifer
Shared with (contributions)
VCMR: Vanderbilt Cryopreserved Mouse Repository Master

Mouse Information

Common Name floxEP4B6
Research Applications Not provided
MMRRC ID Not provided
Jackson Laboratories Stock No 028102
VCMR ID JW
Additional Strain Information Not provided

Genetic Alteration

Mutation #1: Targeted Mutagenesis
Type of AlleleConditional Null
Targeted GeneName: prostaglandin E receptor 4 (subtype EP4)
Symbol: Ptger4
NCBI: 104311
Allele Name: Targeted mutation 1.1
Symbol: Ptger4tm1.1Matb
MGI: 3052967
Description of Targeting Vector Not provided
Vector Genbank File Not provided
Allele Map Not Provided
PCR Genotyping Protocol Breyer_Lab_floxed_EP4_PCR_protocol.pdf
Citations
Publication
Generation of a conditional allele of the mouse prostaglandin EP4 receptor. (2004) Genesis 40: 7-14 (Added 12/21/2013)
PMID: 15354288

Background Strain Information

Strain Type Congenic Strain
Chimera/Founder Genetic Background 129S6/SvEvTac
Current Genetic Background C57BL/6J
Number of Generations Backcrossed >5
Strain Description Not provided

Attachments

Document Breyer_Lab_floxed_EP4_PCR_protocol.pdf - Added on June 18, 2019 at 1:29 PM by Jennifer Skelton


Document floxEP4B6_supporting_info.docx - Added on June 18, 2019 at 1:29 PM by Jennifer Skelton