Floxed mutant mice possessing loxP sites which flank exon 2 of the Ptger4 gene.  

Prostaglandin E2 (PGE2), a fatty acid derivative and a primary target of NSAIDs, is important in diverse physiological processes, including but not limited to: contraction and relaxation of smooth muscle, vasodilation, vasoconstriction, blood pressure regulation, immune response and inflammation regulation and bone formation and healing. There are four receptors for PGE2. The Ptger4 gene encodes a G-protein coupled PGE2 receptor (EP4), which is essential for embryonic development and neonatal survival in mice. 
These mice possess loxP sites on either side of exon 2 of the targeted gene. Mice that are homozygous for this allele are viable and fertile. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissues. Removal of the floxed sequence creates a null allele. During backcrossing, the Y chromosome may not have been fixed to the C57BL/6J genetic background.


Record History
Added on June 18, 2019 at 1:29 PM by Skelton, Jennifer
Modified on June 18, 2019 at 1:49 PM by Skelton, Jennifer
Shared with (contributions)
VCMR: Vanderbilt Cryopreserved Mouse Repository Master
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Mouse Information

Common Name floxEP4B6
VCMR ID JW
Date Cryopreserved 2009-06-24
Method of Cryopreservation Sperm
Trial IVF % Fertilization 25.00%

Genetic Alteration

Mutation #1: Targeted Mutagenesis
Allele Name: Targeted mutation 1.1
Symbol: Ptger3tm1.1Matb
MGI: 3052967
Zygosity at cryopreservation Homozygous
PCR Genotyping Protocol Breyer_Lab_floxed_EP4_PCR_protocol.pdf
Citations
Publication
Generation of a conditional allele of the mouse prostaglandin EP4 receptor. (2004) Genesis 40: 7-14 (Added 12/21/2013)
PMID: 15354288

Background Strain Information

Strain Type Congenic Strain
Chimera/Founder Genetic Background 129S6/SvEvTac
Cryopreservation Strain Background (VCMR) C57BL/6J
Viability and Fertility Data

No known abnormalities.

Attachments

Document Breyer_Lab_floxed_EP4_PCR_protocol.pdf - Added on June 18, 2019 at 1:29 PM by Jennifer Skelton


Document floxEP4B6_supporting_info.docx - Added on June 18, 2019 at 1:29 PM by Jennifer Skelton