This mouse line was generated using both gene targeting and RMCE.  The resulting allele expresses an H2B-Apple fusion protein from the endogenous Ins2 promoter, thereby marking pancreatic beta cells with nuclear-localized red fluorescence.

Record History
Added on December 18, 2018 at 1:31 PM by Skelton, Jennifer
Modified on September 4, 2019 at 1:38 PM by Skelton, Jennifer
Shared with (contributions)
VCMR: Vanderbilt Cryopreserved Mouse Repository Master

Mouse Information

Common Name Ins2.Apple
Research Applications Not provided
MMRRC ID Not provided
Jackson Laboratories Stock No Not provided
Additional Strain Information Not provided

Genetic Alteration

Mutation #1: Targeted Mutagenesis
Type of AlleleCassette Acceptor
Targeted GeneName: Insulin II
Symbol: Ins2
NCBI: 16334
Allele Name: insulin II; targeted mutation 1.1, Mark A Magnuson
Symbol: Ins2tm1.1Mgn
Description of Targeting Vector Not provided
Vector Genbank File Not provided
Allele Map Not Provided
PCR Genotyping Protocol Ins2.Apple.hGH_PCR_Protocol.doc
Transgene-associated human growth hormone expression in pancreatic β-cells impairs identification of sex-based gene expression differences. (2019) Am J Physiol Endocrinol Metab 316: E196-E209 (Added 12/14/2018)
PMID: 30532991

Background Strain Information

Strain Type Congenic Strain
Chimera/Founder Genetic Background 129S6/SvEvTac
Current Genetic Background C57BL/6J
Number of Generations Backcrossed 9
Strain Description Not provided


Document Genetic_Validation_20190219_Summary_Report.pdf - Added on March 29, 2019 at 11:31 AM by Jennifer Skelton

Document Genetic_Validation_Full_Report.pdf - Added on March 29, 2019 at 11:31 AM by Jennifer Skelton