B6.Cg-Ins2{tm1.1Mgn}/Vu - Mouse Strain Resource - Labnodes

This mouse line was generated using both gene targeting and RMCE. The resulting allele expresses an H2B-Apple fusion protein from the endogenous Ins2 promoter, thereby marking pancreatic beta cells with nuclear-localized red fluorescence.

Keywords: Insulin 2 Mgn Apple Ins2 hGH Targeted Mutation

Record History: Added on December 18, 2018 at 1:31 PM by Skelton, Jennifer
Modified on September 4, 2019 at 1:38 PM by Skelton, Jennifer
Shared with (contributions): VCMR: Vanderbilt Cryopreserved Mouse Repository

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Mouse Information

Common Name	Ins2.Apple
VCMR ID	ZI
Date Cryopreserved	2018-12-18
Method of Cryopreservation	Sperm
Trial IVF % Fertilization	68.00%

Genetic Alteration

Mutation #1: Targeted Mutagenesis

Allele

Name: insulin II; targeted mutation 1.1, Mark A Magnuson
Symbol: Ins2^tm1.1Mgn

Zygosity at cryopreservation

Heterozygous

PCR Genotyping Protocol

Ins2.Apple.hGH_PCR_Protocol.doc

Citations

Publication
Transgene-associated human growth hormone expression in pancreatic β-cells impairs identification of sex-based gene expression differences. (2019) Am J Physiol Endocrinol Metab 316: E196-E209 (Added 12/14/2018) PMID: 30532991

Background Strain Information

Strain Type	Congenic Strain
Chimera/Founder Genetic Background	129S6/SvEvTac
Cryopreservation Strain Background (VCMR)	C57BL/6J
Viability and Fertility Data	Homozygous litters are viable and fertile. Backcrossed 9 generations onto C57Bl6/J.