This mouse line was generated using both gene targeting and RMCE.  The resulting allele expresses an H2B-Apple fusion protein from the endogenous Ins2 promoter, thereby marking pancreatic beta cells with nuclear-localized red fluorescence.


Record History
Added on December 18, 2018 at 1:31 PM by Skelton, Jennifer
Modified on March 19, 2019 at 11:12 AM by Skelton, Jennifer
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VCMR: Vanderbilt Cryopreserved Mouse Repository Master
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Mouse Information

Common Name Ins2.Apple
VCMR ID ZI
Date Cryopreserved 2018-12-18
Method of Cryopreservation Sperm
Trial IVF % Fertilization 68.00%

Genetic Alteration

Mutation #1: Targeted Mutagenesis
Allele Name: Targeted mutation 1.1
Symbol: Ins2tm1.1Mgn
Zygosity at cryopreservation Heterozygous
PCR Genotyping Protocol Ins2.Apple.hGH_PCR_Protocol.doc
Citations
Publication
Transgene-associated human growth hormone expression in pancreatic β-cells impairs identification of sex-based gene expression differences. (2019) Am J Physiol Endocrinol Metab 316: E196-E209 (Added 12/14/2018)
PMID: 30532991

Background Strain Information

Strain Type Congenic Strain
Chimera/Founder Genetic Background 129S6/SvEvTac
Cryopreservation Strain Background (VCMR) C57BL/6J
Viability and Fertility Data

Homozygous litters are viable and fertile.

Backcrossed 9 generations onto C57Bl6/J.