Start Date / Time | January 16, 2019 at 9:00 AM |
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End Date / Time | January 16, 2019 at 10:00 AM |
Duration | 1 hour(s) |
Location | 9455 MRB IV |
Presenter Name | Chris Wright, D. Phil. |
Presentation Title | Barcoding of mammalian cells using homing CRISPR |
Status | This meeting has already occurred |
Rationale:
Occasional presentations by faculty mentors regarding paradigm-shifting discoveries or enabling techniques in areas related to stem and progenitor biology could be useful to the VCSCB community.
Coverage:
Combinatorial barcoding may provide an essential and refined level of understanding of lineage connections in many biological and disease contexts. I aim to guide discussion over Kalhor et al. ([Church Lab] Science 2018; doi 10.1126/science.aat9804), which describes homing-guide RNA/CRISPR-based barcode lineage-labeling of millions of cells in mammalian embryos. Kalhor’s 60-insert hgRNA transgenic mouse strain may be bred to constitutive, tissue-specific, or inducible iCAS expressers, for flexible tracing approaches with minimal derivation of new strains. I will include a speculative proposal from Gaj and Perez-Pinera (Genome Biology 2018; doi 10.1186/s13059-018-1541-y) for using EvolvR (Halperin et al. [Dueber lab] Nature 2018; doi 10.1038/s41586-018-0384-8 –– an nCas9 variant fused to error-prone DNA polymerase) to generate greater barcode diversity, potentially from fewer hgRNA insert sites. I hope for active discussion of applications and limitations.
Serendipity: While I selected this paper many months ago for this SPRING presentation, please note that Dr. Reza Kalhor (first author) will visit us as a CDB faculty candidate on Jan 24/25, 2019.
Spring_2019_Email_Notice_Wright.pdf - Added on January 14, 2019 at 11:24 AM by Pam Uttz