|Start Date / Time||January 16, 2019 at 9:00 AM|
|End Date / Time||January 16, 2019 at 10:00 AM|
|Location||9455 MRB IV|
|Presenter Name||Chris Wright, D. Phil.|
|Presentation Title||Barcoding of mammalian cells using homing CRISPR|
|Status||This meeting is currently scheduled|
Occasional presentations by faculty mentors regarding paradigm-shifting discoveries or enabling techniques in areas related to stem and progenitor biology could be useful to the VCSCB community.
Combinatorial barcoding may provide an essential and refined level of understanding of lineage connections in many biological and disease contexts. I aim to guide discussion over Kalhor et al. ([Church Lab] Science 2018; doi 10.1126/science.aat9804), which describes homing-guide RNA/CRISPR-based barcode lineage-labeling of millions of cells in mammalian embryos. Kalhor’s 60-insert hgRNA transgenic mouse strain may be bred to constitutive, tissue-specific, or inducible iCAS expressers, for flexible tracing approaches with minimal derivation of new strains. I will include a speculative proposal from Gaj and Perez-Pinera (Genome Biology 2018; doi 10.1186/s13059-018-1541-y) for using EvolvR (Halperin et al. [Dueber lab] Nature 2018; doi 10.1038/s41586-018-0384-8 –– an nCas9 variant fused to error-prone DNA polymerase) to generate greater barcode diversity, potentially from fewer hgRNA insert sites. I hope for active discussion of applications and limitations.
Serendipity: While I selected this paper many months ago for this SPRING presentation, please note that Dr. Reza Kalhor (first author) will visit us as a CDB faculty candidate on Jan 24/25, 2019.