Natalya Ortolano
Graduate Student (Vivian Gama Lab)

Record History
Added on August 31, 2018 at 2:18 PM by Uttz, Pam
Modified on November 30, 2018 at 10:17 AM by Uttz, Pam
Shared with (contributions)
Public: SPRING Meeting

Meeting Details

Start Date / Time December 5, 2018 at 9:00 AM
End Date / Time December 5, 2018 at 10:00 AM
Duration 1 hour(s)
Location 9455 MRB IV
Presenter Name Natalya Ortolano (Gama Lab)
Presentation Title Determining the functional role of CUL9 during the early stages of human corticogenesis
Status This meeting has already occurred

Meeting Agenda/Notes

The cullin-ring ligase (CRL) family of E3 ubiquitin ligases plays a critical role in regulation of early developmental processes, including synapse formation and axon guidance. However, Cullin-9 (CUL9) has proven to be a unique CRL with an elusive function. My data suggest a role for CUL9 in the early neural induction phase of cortical differentiation. CUL9 protein levels are induced during cortical glutamatergic differentiation. Interestingly, knockdown of CUL9 protein levels by lentiviral transduction in human pluripotent stem cells (hPSCs) caused abnormal cortical differentiation. Specifically, hPSCs with depleted CUL9 levels formed enlarged embryoid bodies and fewer neural rosettes with expanded lumenal areas. This phenotype was accompanied by elevated PAX6 levels, a transcription factor key in regulating hNPC properties, in CUL9 knockdown human neural precursor cells (hNPCs) and cortical glutamatergic neurons. We hypothesize that CUL9 may be critical for proper neural rosette formation and subsequent neurogenesis through its regulation of substrates key for neural cell fate.



Document Fall_2018_Email_Notice_Ortolano.pdf - Added on November 30, 2018 at 10:17 AM by Pam Uttz