|Start Date / Time||October 24, 2018 at 9:00 AM|
|End Date / Time||October 24, 2018 at 10:00 AM|
|Location||9455 MRB IV|
|Presenter Name||Xiaodong Zhu, Ph.D. (Gannon Lab)|
|Presentation Title||Regulation of endocrine specification by Oc1 and Pdx1|
|Status||This meeting has already occurred|
Previous work in our lab has demonstrated that Pdx1 and Oc1 cooperatively regulate pancreas endocrine development. To further understand the regulation of endocrine cell specification by Oc1 and Pdx1, we use over-expression strategies in cultured human pancreatic ductal epithelial cells, and heterozygous loss of function studies with wholemount immunofluorescence of e11.5 mouse embryos. Our preliminary results show that Pdx1+ cells undergoing mitosis have lower levels of Pdx1 protein than non-dividing pancreatic epithelial cells. Overexpression of Pdx1 causes mitotic defects including multipolar spindles and anaphase lagging chromosomes. We hypothesize that Pdx1 is not only essential for cell proliferation, but needs also to be tightly regulated throughout the cell cycle. Additionally, in cells weakly expressing Pdx1 that have undergone a brief period of Oc1 expression, we are able to detect glucagon mRNA and Ngn3 and MafB protein, suggesting that the combined expression of Pdx1 and Oc1 may be able to reprogram duct cells into endocrine cells.