PDF

SPRING Meeting

 

James Patton, Ph.D.
Professor of Biological Sciences, Cell and Developmental
Biology and Ophthalmology and Visual Sciences
Stevenson Chair of Biological Sciences


 

Record History
Added on August 31, 2018 at 2:12 PM by Uttz, Pam
Modified on October 5, 2018 at 10:27 AM by Uttz, Pam
Shared with (contributions)
Public: SPRING Meeting

Meeting Details

Start Date / Time October 10, 2018 at 9:00 AM
End Date / Time October 10, 2018 at 10:00 AM
Duration 1 hour(s)
Location 9455 MRB IV
Presenter Name James Patton, Ph.D.
Presentation Title The miR-216a-Dot1l regulatory axis is necessary and sufficient for Müller glia reprogramming during retina regeneration
Status This meeting has already occurred

Meeting Agenda/Notes

Unlike the adult mammalian retina, Müller glia (MG) in the adult zebrafish retina are able to dedifferentiate into a ‘stem cell’-like state and give rise to multipotent progenitor cells upon retinal damage.  We show that miR-216a is downregulated in MG after constant intense light lesioning and that miR-216a suppression is necessary and sufficient for MG dedifferentiation and proliferation during retina regeneration.  miR-216a targets the H3K79 methyltransferase Dot1l which is upregulated in proliferating MG after retinal damage. Loss-of-function experiments show that Dot1l is necessary for MG reprogramming and mediates MG proliferation downstream of miR-216a.  We further demonstrate that miR-216a and Dot1l regulate MG-mediated retina regeneration through canonical Wnt signaling.  Together, our study reports a novel regulatory mechanism upstream of Wnt signaling during retina regeneration and provides potential targets for enhancing regeneration in the adult mammalian retina.

Attachment

Document Fall_2019_Email_Notice_Patton.pdf - Added on October 5, 2018 at 10:27 AM by Pam Uttz