Using CRISPR, the codon encoding Lys21 in Dnpep, exon 2, was modified to encode a stop codon. Additional mutations were introduced to create a BspHI restriction site for genotyping purposes and protect the repaired DNA from sgRNA-mediated cas9 targeting.  


Record History
Added on August 3, 2018 at 9:27 AM by Skelton, Jennifer
Modified on December 6, 2018 at 9:37 AM by Skelton, Jennifer
Shared with (contributions)
VCMR: Vanderbilt Cryopreserved Mouse Repository Master

Mouse Information

Common Name Dnpep
Research Applications Not provided
MMRRC ID Not provided
Jackson Laboratories Stock No Not provided
VCMR ID ZB
Additional Strain Information Not provided

Genetic Alteration

Mutation #1: Nuclease-Mediated
Allele Name: aspartyl aminopeptidase; endonuclease mediated mutation 1, Eric Delpire
Symbol: Dnpepem1Dlp
MGI: 6120903
PCR Genotyping Protocol Dnpep_Genotyping_Protocol.docx
Citations
Publication
DNPEP is not the only peptidase that produces SPAK fragments in kidney. (2017) Physiol Rep 5: (Added 2/15/2018)
PMID: 29122955

Background Strain Information

Strain Type Recombinant Inbred
Chimera/Founder Genetic Background B6D2
Current Genetic Background C57BL/6J
Number of Generations Backcrossed 8
Strain Description Not provided

Attachment

Document Delpire_Dnpep.PDF - Added on August 3, 2018 at 9:27 AM by Jennifer Skelton