The pLCA.66.2272.FRTs plasmid is modified to include EM7-Neo at EcoR1 site for future use.

Cut out EN7-Neo from pLCA.66.2272 using EcoRI and ligate at EcoR1 site just before 3'FRT. After that Neo insertion clone the 5' FRT from PL451.FRT4.XE6 plasmid at blunted HindIII after lox 66 site.

Sequence confirm the clones that are screened by restriction analysis.

 

 


Category
Plasmid
Record History
Added on March 20, 2012 at 10:36 AM by Gangula, Rama
Modified on March 20, 2012 at 10:36 AM by Gangula, Rama
Shared with (contributions)
VCSCBi: Training / Vectors

Vector Annotations

Vector Map BEOOduwH.png
Genbank File pLCA.66.2272.Neo.FRTs.gb
Backbone Vector phygro66.2272
Construct Size 6,838 bp (approximate)
Bacterial Stock No
Storage Temperature -20°C
Stock Concentration 0.1 µg/µL
Addgene Not Provided

Source

Laboratory Mark Magnuson
Made byRama Gangula
Stock Date March 20, 2012

Inventory Location

Private