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First exon of Efna1 gene which encodes the EphrinA1 was replaced with a neo cassette.  The absence of protein expression was confirmed by Western blot analysis of P0 lung extracts.

Keywords: Efna1 Ephrin-A1

Record History
Added on April 17, 2018 at 11:18 AM by Skelton, Jennifer
Modified on April 17, 2018 at 11:26 AM by Skelton, Jennifer
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VCMR: Vanderbilt Cryopreserved Mouse Repository Master
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Mouse Information

Common Name Efna1 KO/BalbC
VCMR ID OJ
Date Cryopreserved 2012-10-03
Method of Cryopreservation Sperm
Trial IVF % Fertilization 78.00%

Genetic Alteration

Mutation #1: Targeted Mutagenesis
Allele Name: ephrin A1; targeted mutation 1, Jin Chen
Symbol: Efna1tm1Jinc
MGI: MGI:4849544
Zygosity at cryopreservation Homozygote
PCR Genotyping Protocol Efna1_KO_PCR_Protocol.docx
Citations
Publication
Regulation of heart valve morphogenesis by Eph receptor ligand, ephrin-A1. (2010) Dev Dyn 239: 3226-34 (Added 11/13/2013)
PMID: 20960543

Background Strain Information

Strain Type Congenic Strain
Chimera/Founder Genetic Background 129X1/SvJ
Cryopreservation Strain Background (VCMR) BALB/c
Viability and Fertility Data

Backcrossed greater than 10 times at cryopreservation to the BALB/C background.

Viable and Fertile; Average litter size is around 6 pups.