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Reagents, supplies, and equipment: 

  • Paper clip for plunger
  • IVF dish
  • Scissors
  • 100% ethanol
  • Room temperature water bath

Category
Protocol
Record History
Added on March 7, 2018 at 3:24 PM by Skelton, Jennifer
Modified on March 7, 2018 at 3:26 PM by Skelton, Jennifer
Shared with (contributions)
VCMR: Recovery Protocols Master

Thawing Sperm in Straws:

  1. Remove straw from LN2 storage.
  2. Immerse straw in room temperature water bath until all ice crystals are melted, approximately 2 minutes.
  3. Wipe of straw with ethanol.
  4. Holding the straw firmly, cut off the seal, leaving about half of the cotton plug in place.
  5. Using a metal rod as a plunger, push against the remaining portion of the cotton plug and expel the sperm fraction into dish of IVF media.