Reagents, supplies, and equipment:

  • Wide bore pipette tips

Category
Protocol
Record History
Added on March 7, 2018 at 3:19 PM by Skelton, Jennifer
Modified on March 7, 2018 at 3:28 PM by Skelton, Jennifer
Shared with (contributions)
VCMR: Recovery Protocols Master

Thawing Sperm in Vials:

  1. Remove the cryo vial from LN2 storage. Place the vial at room temperature until thawed, about 12 to 15 minutes.
  2. Using a wide bore pipette tip, remove quantity of sperm needed for IVF.