|Start Date / Time||April 5, 2017 at 9:00 AM|
|End Date / Time||April 5, 2017 at 10:00 AM|
|Location||9455 MRB IV|
|Presenter Name||Gregor Neuert, Ph.D.|
|Presentation Title||Temporal control of cellular phenotypes|
|Status||This meeting has already occurred|
Many biological processes are driven by gradually and temporally changing environments resulting in cell signaling and gene expression responses which are often malfunction in human diseases. How cells sense, transmit and phenotypically respond to these temporally changing environmental signals in comparison to rapidly or acute changing environment is mostly unknown. Here we demonstrate that a repetitive linear stress gradient but not a step, pulse or a quadratic gradient of the same intensity causes a severe cell growth phenotype. Although single cells physically sense the stress gradient, they are not protected from such a temporal stress. We identified a gradient sensing threshold as the mechanism that prevent the Stress Activated Protein Kinase (p38 / Hog1 SAPK) pathway to active single cell signal transduction, single cell mRNA transcription and lack of cellular osmolyte production. We demonstrate that cells can be protected against such a temporal stress through short and prior induction of permanently phosphorylated SAPK. Our approach is general because it allows to interrogate biological phenotypes under physiologically relevant temporal conditions.