|Start Date / Time||March 22, 2017 at 9:00 AM|
|End Date / Time||March 22, 2017 at 10:00 AM|
|Location||9455 MRB IV|
|Presenter Name||Karrie Dudek (Magnuson Lab)|
|Presentation Title||Towards an understanding of the gene regulatory network of pancreatic pro-endocrine progenitor cells|
|Status||This meeting has already occurred|
This presentation will describe two related studies that seek to accelerate our understanding of the gene regulatory network in nascent pancreatic endocrine cells. First, I will present our preliminary findings from applying differential gene expression analysis to RNA-Seq datasets isolated from pre-endocrine cells devoid of either NeuroD1, Insm1 or Ripply3. Second, I will present data describing our use of the Tol2 transposase system in combination with Cas9-expressing mice to impair gene expression in a manner that goes beyond existing Cre/LoxP methods.