|Jackson Laboratories Stock No||Not provided|
|Additional Strain Information||Not provided|
|Mutation #1: RMCE Targeted Mutagenesis|
|Type of Allele||Cassette Acceptor|
|Targeted Gene||Name: gene trap ROSA26, Philippe Soriano|
Name: targeted mutation 1
|Description of Targeting Vector||
This mouse line contains a loxed cassette acceptor (LCA) allele in which a 5.17 kb region of the gene has been replaced by a lox71 site, a puromycin-(delta)-thymidine kinase fusion gene driven by the mouse phosphoglycerol kinase promoter, a kanamycin resistance gene driven by the bacterial EM7 promoter, and a lox2272 site. These features enable use of Recombinase-Mediated Cassette Exchange (RMCE) for the rapid insertion of various DNAs into the Rosa26 gene locus.
|Vector Genbank File||
|Allele Map||Not Provided|
|PCR Genotyping Protocol||Not provided|
|Type of Allele||Global Null|
|Exchanged Cassette Gene Name|| ()
|Exchanged Cassette Allele Name||pRosa.EN.CFP.bGsplicepA.Neo
|Description of Exchange Vector||
The pRosa.EN.CFP.bGsplicepA.Neo exchange vector was made on a backbone of a basal exchange vector which contains a 4.081 kb sequence from the Rosa26 locus, Lox71/Lox2272 sites, and a flrted (flanked by FRT) Pgk-Neo cassette that is used for positive selection of ES cells after RMCE. A 5' translational enhancer-CFP-intron-containing rabbit beta-globin polyA sequence was inserted (in place of Rosa26 exon 1), between the Lox71 and the Pgk-Neo sites.
|Exchanged Cassette Genbank File||
|PCR Genotyping Protocol||
|Chimera/Founder Genetic Background||129S6/SvEvTac|
|Current Genetic Background||C57BL/6J|
|Number of Generations Backcrossed||4|
Founder chimeras were crossed with C57Bl/6J for 4 generations.
The images of newborn offspring from intercrosses of Rosa26Cherry/ ,Rosa26CFP/ and Rosa26YFP/ mice were taken with a stereoscope using CFP, YFP and RFP filter and subsequently overlayed.
The image of Rosa26CFP/ mouse was taken with a stereoscope using CFP filter.