|Start Date / Time||May 17, 2016 at 10:00 AM|
|End Date / Time||May 17, 2016 at 10:55 AM|
|Location||512 Light Hall|
|Presenter Name||Christian Meyer and David Wooten (VQuaranta’s lab)|
|Presentation Title||Engineering Endocrine Transdifferentiation: A Systems Approach|
|Status||This meeting has already occurred|
Understanding how cellular identity emerges from underlying gene regulatory networks (GRNs) is challenging, yet recent studies have demonstrated that cellular identity can be controlled by targeted activation or silencing of a subset of key transcription factors (TFs). However, identifying these key TFs remains challenging because of the number of possible combinations of network perturbations. Here we propose a method which leverages the global-topology information of an endocrine-specific GRN to come up with computationally predicted and unsuspected methods for engineering alpha to beta cell transdifferentiation. Preliminary analysis predicts that aberrant expression/knockdown of three critical TFs: PAX4, MAFB, and cJUN, will induce alpha to beta cell conversion in the murine model.