|Jackson Laboratories Stock No||Not provided|
|Additional Strain Information||Not provided|
|Mutation #1: RMCE Targeted Mutagenesis|
|Type of Allele||Cassette Acceptor|
|Targeted Gene||Name: gene trap ROSA26, Philippe Soriano|
Name: Targeted mutation 1
|Description of Targeting Vector||
This ES cell line contains a loxed cassette acceptor (LCA) allel in which a 5.17kb region of the gene has been replaced by a lox71 site, a puromycin-(delta)-thymidine kinase fusion gene driven by the mouse phosphoglycerol kinase promoter, a kanamycin resistance gene driven by the bacterial EM7 promoter, and a lox2272 site. These features enable use of Recombinase-Mediated Cassette Exchange (RMCE) for the rapid insertion of various DNAs into the Rosa26 gene locus.
|Vector Genbank File||
|Allele Map||Not Provided|
|PCR Genotyping Protocol||Not provided|
|Type of Allele||Global Null|
|Exchanged Cassette Gene Name|| ()
|Exchanged Cassette Allele Name||YFP
|Description of Exchange Vector||
The pRosa.EN.YFP.bGsplicepA.Neo vector was made on a backbone of a basal exchange vector which contains a 4.081 kb sequence from the Rosa26 locus, Lox71/Lox2272 sites, and a flrted (flanked by FRT) Pgk-Neo cassette that is used for positive selection of ES cells after RMCE. A 5' translational enhancer-YFP- intron-containing rabbit beta-globin polyA sequence was inserted (in place of Rosa26 exon 1), between the Lox71 and Pgk-Neo sites.
|Exchanged Cassette Genbank File||
|PCR Genotyping Protocol||
|Chimera/Founder Genetic Background||129S6/SvEvTac|
|Current Genetic Background||C57BL/6J|
|Number of Generations Backcrossed||3|
After germline transmission, founder chimeras 129S6 were bred to C57Bl/6J mice for 3 generations.