This mouse line was made by RMCE in the Rosa26.LCA allele and exhibits yellow fluorescent protein (YFP, Citrine) expressed under control of the ROSA26 promoter.  The YFP sequence is preceded by a translational enhancer and is followed by intron-containing rabbit b-globin polyA sequences.  This mouse ubiquitously expresses YFP protein and can be used for transplantation and other experiments where tracing of source cells is required.

 

 


Record History
Added on September 30, 2011 at 12:57 PM by Peters, Jody
Modified on April 13, 2017 at 2:53 PM by Skelton, Jennifer
Shared with (contributions)
Public: Shared Collection

Mouse Information

Common Name Rosa26YFP
Research Applications Fluorescent proteins
MMRRC ID 036289-MU
Jackson Laboratories Stock No Not provided
VUCMR ID NJ
Additional Strain Information Not provided

Genetic Alteration

Mutation #1: RMCE Targeted Mutagenesis
Type of AlleleCassette Acceptor
Targeted GeneName: gene trap ROSA26, Philippe Soriano
Symbol: Gt(ROSA)26Sor
NCBI: 14910
Allele Name: Targeted mutation 1
Symbol: Rosa26tm1(LCA)
MGI: 5287870
Description of Targeting Vector

This ES cell line contains a loxed cassette acceptor (LCA) allel in which a 5.17kb region of the gene has been replaced by a lox71 site, a puromycin-(delta)-thymidine kinase fusion gene driven by the mouse phosphoglycerol kinase promoter, a kanamycin resistance gene driven by the bacterial EM7 promoter, and a lox2272 site.  These features enable use of Recombinase-Mediated Cassette Exchange (RMCE) for the rapid insertion of various DNAs into the Rosa26 gene locus.


Vector Genbank File pRosa26.LCA.gb
Allele Map Not Provided
PCR Genotyping Protocol Not provided
Type of Allele Global Null
Exchanged Cassette Gene Name ()
Exchanged Cassette Allele Name YFP
Description of Exchange Vector

The pRosa.EN.YFP.bGsplicepA.Neo vector was made on a backbone of a basal exchange vector which contains a 4.081 kb sequence from the Rosa26 locus, Lox71/Lox2272 sites, and a flrted (flanked by FRT) Pgk-Neo cassette that is used for positive selection of ES cells after RMCE.  A 5' translational enhancer-YFP- intron-containing rabbit beta-globin polyA sequence was inserted (in place of Rosa26 exon 1), between the Lox71 and Pgk-Neo sites.

Exchanged Cassette Genbank File pRosa.EN.YFP.bGsplicepA.neo.gb
Citations
Publication
Quantification of factors influencing fluorescent protein expression using RMCE to generate an allelic series in the ROSA26 locus in mice. (2011) Dis Model Mech 4: 537-47 (Added 11/8/2013)
PMID: 21324933

Background Strain Information

Strain Type Mixed
Chimera/Founder Genetic Background 129S6/SvEvTac
Current Genetic Background C57BL/6J
Number of Generations Backcrossed 3
Strain Description

After germline transmission, founder chimeras 129S6 were bred to C57Bl/6J mice for 3 generations.

Attachment

Document R26_EN_YFP_RMCEs.png - Added on February 7, 2012 at 1:44 PM by Jody Peters

Linear Map of Exchange Event