Current and ongoing beta cell research is presented in this weekly seminar by faculty, postdoctoral fellows and students. If you are interested in attending the Beta Cell Interest Group (BIG) seminars and joining the BIG community, please contact David Jacobson.


Record History
Added on November 4, 2015 at 8:56 AM by Brown, Deborah
Modified on February 26, 2016 at 9:44 AM by Brown, Deborah
Shared with (contributions)
BIG: Beta Cell Interest Group (BIG) seminar Master

Meeting Details

Start Date / Time March 2, 2016 at 9:00 AM
End Date / Time March 2, 2016 at 9:55 AM
Duration 55 minutes
Location 512 Light Hall
Presenter Name Anna Osipovich (Mark Magnuson's lab)
Presentation Title A gene co-expression network of beta-cell development
Status This meeting has already occurred

Meeting Agenda/Notes

I will describe a collaborative effort that resulted in the generation of a comprehensive lineage-directed gene correlation network for mouse beta-cells.  This undertaking utilized a total of approximately 1.5 billion mapped sequence reads from 33 RNA-Seq datasets representing eleven different FACS-purified murine cell populations, each of which were temporally and genetically defined.  Our study required the development of iterative WGCNA approach which, in the end, enabled us to classify 10,721 of the 15,949 genes expressed in these cell populations into 79 modules. We further grouped these modules using community detection analysis into 14 meta-modules.  Together, this effort resulted in an easily interpretable framework for the development of pancreatic beta-cells and related functional predictions.