This mouse lines expresses mCherry, a red flourescent protein, under control of the endogenous ROSA26 gene locus.  This mouse was generated as part of a study to identify the optimal combination of regulatory elements for flourescent protein expression from a single gene copy.

 


Record History
Added on July 12, 2011 at 10:52 AM by Peters, Jody
Modified on April 13, 2017 at 2:30 PM by Skelton, Jennifer
Shared with (contributions)
Public: Shared Collection

Mouse Information

Common Name Rosa26EN.Cherry.Neo
Research Applications Fluorescent proteins
MMRRC ID 036286-UCD
Jackson Laboratories Stock No Not provided
VUCMR ID NI
Additional Strain Information Not provided

Genetic Alteration

Mutation #1: RMCE Targeted Mutagenesis
Type of AlleleCassette Acceptor
Targeted GeneName: gene trap ROSA 26, Philippe Soriano
Symbol: Gt(ROSA)26Sor
NCBI: 14910
Allele Name: targeted mutation 1
Symbol: Rosa26tm1(LCA)
MGI: 5085307
Description of Targeting Vector

This mouse line contains a loxed cassette acceptor (LCA) allele in which a 5.17 kb region of the gene has been replaced by a lox71 site, a puromycin-(delta)-thymidine kinase fusion gene driven by the mouse phosphoglycerol kinase promoter, a kanamycin resistance gene driven by the bacterial EM7 promoter, and a lox2272 site.  These features enable use of Recombinase-Mediated Cassette Exchange (RMCE)  for the rapid insertion of various DNAs into the the Rosa26 gene locus. 


Vector Genbank File TargetVectorGenebankpRosa26.LCA.gb
Allele Map Not Provided
PCR Genotyping Protocol Not provided
Type of Allele Gene Replacement
Exchanged Cassette Gene Name ()
Exchanged Cassette Allele Name pRosa.EN.Cherry.bGsplicepA.Neo
Description of Exchange Vector

Not Provided

Exchanged Cassette Genbank File ExchangeVectorpRosa.EN.Cherry.bGsplicepA.neo.gb
Citations
Publication
Quantification of factors influencing fluorescent protein expression using RMCE to generate an allelic series in the ROSA26 locus in mice. (2011) Dis Model Mech 4: 537-47 (Added 11/8/2013)
PMID: 21324933

Background Strain Information

Strain Type Mixed
Chimera/Founder Genetic Background 129S6/SvEvTac
Current Genetic Background C57BL/6J
Number of Generations Backcrossed 2
Strain Description

This strain is of a mixed genetic background that is approximately 25% 129S6 and 75% C57BL6/J.

Attachment

Document LinearMapr26encherry.png - Added on July 12, 2011 at 10:52 AM by Jody Peters

This figure shows how this line of mice was made. Coding sequences for a red (mCherry) flourescent protein gene were inserted into an exchange cassette that allowed RMCE into a ROSA26 [LCA] allele. In this manner, mCherry is constitutively expressed under control of the endogenous ROSA26 promoter. The exchange plasmid also contains a 51bp translational enhancer (5' leader sequence from Xenopus beta-globin), a Kozak sequence upstream of the start codon, and intronic an polyA sequences from the rabbit beta-globin gene that confer stability to the mRNA.