This mouse line expresses the reverse tetracycline transactivator (rtTA) under control of the Ptf1a gene.  It can be used to activate expression of TetO-regulated genes in sites where PTF1a is expressed, such as pancreatic acinar cells.

 


Record History
Added on July 12, 2011 at 9:30 AM by Peters, Jody
Modified on April 14, 2017 at 11:18 AM by Skelton, Jennifer
Shared with (contributions)
Public: Shared Collection

Mouse Information

Common Name Ptf1artTA
Research Applications Tet expression systems
MMRRC ID 036492-Jax
Jackson Laboratories Stock No Not provided
VUCMR ID LO, LP
Additional Strain Information Not provided

Genetic Alteration

Mutation #1: RMCE Targeted Mutagenesis
Type of AlleleGene Replacement
Targeted GeneName: pancreas specific transcription factor, 1a
Symbol: Ptf1a
NCBI: 19213
Allele Name: targeted mutation 2
Symbol: Ptf1atm1.2(rtTA)Mgn
MGI: 5467924
Description of Targeting Vector

Not Provided


Vector Genbank File Ptf1a.LCA.gb
Allele Map Not Provided
PCR Genotyping Protocol Not provided
Type of Allele
Exchanged Cassette Gene Name (19213)
Exchanged Cassette Allele Name Ptf1a{LCA}
Description of Exchange Vector

Not Provided

Exchanged Cassette Genbank File ptf1a.rtta.hygro_1.gb
Citations Not provided

Background Strain Information

Strain Type Mixed
Chimera/Founder Genetic Background 129S6/SvEvTac
Current Genetic Background C57BL/6
Number of Generations Backcrossed 6
Strain Description

This line is in a mixed genetic background containing both 129S6 and C57Bl/6 DNA

Publications / Citations

  1. Pancreatic Inflammation Redirects Acinar to β Cell Reprogramming. Clayton HW, Osipovich AB, Stancill JS, Schneider JD, Vianna PG, Shanks CM, Yuan W, Gu G, Manduchi E, Stoeckert CJ, Magnuson MA (2016) Cell Rep 17(8): 2028-2041
    › Primary publication · 27851966 (PubMed) · PMC5131369 (PubMed Central) · Added on 11/18/2016

Attachments

Document linear_map_of_targeting_of_ptf1artta1.png - Added on July 12, 2011 at 9:50 AM by Jody Peters

This figure shows how this line of mice was made. Coding sequences for the reverse tetracycline transactivator (rtTA) were inserted into an exchange cassette that allowed RMCE into a Ptf1a[LCA] allele. In this manner, rtTA is expressed under control of the endogenous Ptf1a promoter. The exchange plasmid also contains a 51bp translational enhancer (5' leader sequence from Xenopus beta-globin gene), a Kozak sequence upstream of the start condon, and intronic and polyA sequences from the rabbit beta-globin gene that confer stability to the mRNA. The hygromycin resistence cassette, necessary for RMCE, was removed by intercrossing the FlpE-expressing mice then segregating the two alleles.


Document Ptf1a.rtTA_hygro_PCR.docx - Added on August 30, 2013 at 2:39 PM by Jody Peters

PCR protocol for Ptf1a.rtTA