|Start Date / Time||November 18, 2015 at 9:00 AM|
|End Date / Time||November 18, 2015 at 10:00 AM|
|Location||9455 MRB IV|
|Presenter Name||Adrian Cadar|
|Presentation Title||Real-time visualization of endogenous titin dynamics in human induced pluripotent stem cell-derived cardiomyocytes|
|Status||This meeting has already occurred|
The giant myofilament protein titin is a critical determinant of myofibril elasticity and sarcomere structure in striated muscle. Although significant advances have been made in our understanding of the biophysical and mechanical properties of titin, the mechanisms governing titin turnover are poorly understood. I will present work regarding the generation of a novel hiSPC line via CRISPR/Cas9 gene editing that allows for the direct visualization of endogenous titin incorporation and turnover in the sarcomeric complex in beating human cardiomyocytes.