Anna Osipovich, Ph.D.
Research Instructor
Magnuson Lab


Record History
Added on September 15, 2015 at 1:01 PM by Uttz, Pam
Modified on October 5, 2015 at 10:14 AM by Uttz, Pam
Shared with (contributions)
Public: SPRING Meeting

Meeting Details

Start Date / Time October 7, 2015 at 9:00 AM
End Date / Time October 7, 2015 at 10:00 AM
Duration 1 hour(s)
Location 9455 MRB IV
Presenter Name Anna Osipovich, Ph.D.
Presentation Title A gene co-expression network of β-cell development
Status This meeting has already occurred

Meeting Agenda/Notes

I will describe a collaborative effort that resulted in the generation of a comprehensive lineage-directed gene correlation network for mouse β-cells.  This undertaking utilized a total of approximately 1.5 billion mapped sequence reads from 33 RNA-Seq datasets representing eleven different FACS-purified murine cell populations, each of which were temporally and genetically defined.  Our study required the development of iterative WGCNA approach which, in the end, enabled us to classify 10,721 of the 15,949 genes expressed in these cell populations into 79 modules. We further grouped these modules using community detection analysis into 14 meta-modules.  Together, this effort resulted in an easily interpretable framework for the development of pancreatic β-cells and related functional predictions.