This mouse line was made by gene targeting and designed to express an HA-tagged version of the transcription factor Neurog3 (Ngn3). The Lox71 and Lox2272 sites were inserted flanking the Ngn3 coding sequence. Within the flanked region an HA-tag was placed at the N-terminus of the Ngn3 protein. This allele can be used to study chromatin and protein-protein interactions of Ngn3. Additionally, the Lox71 and Lox2272 sites allow for manipulations of the flanked region by Recombinase-Mediated Cassete Exchange (RMCE) in ES cells.

 


Record History
Added on July 7, 2015 at 10:27 AM by Magnuson, Mark
Modified on September 5, 2019 at 1:56 PM by Skelton, Jennifer
Shared with (contributions)
VCMR: Vanderbilt Cryopreserved Mouse Repository

Mouse Information

Common Name Neurog3(HA.LCA)
Research Applications Not provided
MMRRC ID NA
Jackson Laboratories Stock No NA
VCMR ID XG
Additional Strain Information

Cryopreserved in homozygous state Neurog3{(HA/HA)}.

The targeting vector contains a 5.5 kb 5’ arm and a 3.5 kb 3’arm.  Lox71 and Lox2272 sites were inserted flanking the Ngn3 gene, where Ngn3 cDNA was replaced with that encoding for an HA-tagged Ngn3 protein.  The vector also contains a FRT-flanked Pgk-Puro-deltaTK cassette for positive selection by puromycin (targeting events) and negative selection by ganciclovir (RMCE events).

Genetic Alteration

Mutation #1: Targeted Mutagenesis
Type of AlleleCassette Acceptor
Targeted GeneName: Neurogenin 3
Symbol: Neurog3
NCBI: 11925
Allele Name: neurogenin 3; targeted mutation, Guoqiang Gu
Symbol: Neurog3(HA.LCA)Ggu
Description of Targeting Vector

The targeting vector contains a 5.5 kb 5’ arm and a 3.5 kb 3’arm.  Lox71 and Lox2272 sites were inserted flanking the Ngn3 gene, where Ngn3 cDNA was replaced with that encoding for an HA-tagged Ngn3 protein.  The vector also contains a FRT-flanked Pgk-Puro-deltaTK cassette for positive selection by puromycin (targeting events) and negative selection by ganciclovir (RMCE events).


Vector Genbank File Ngn3HALCA.gb
Allele Map Not Provided
PCR Genotyping Protocol Neurog3.HA_PCR_genotyping_protocol.docx
Citations Not provided

Background Strain Information

Strain Type Congenic Strain
Chimera/Founder Genetic Background 129S6/SvEvTac
Current Genetic Background CD-1
Number of Generations Backcrossed Not provided
Strain Description

Strain Background:  90% CD-1 at cryopreservation

 

 

Attachments

Document Magnuson_Gu_Ngn3.HA.LCA_targeting.pptx - Added on February 15, 2018 at 10:47 AM by Jennifer Skelton


Document Ngn3_HA_staining.png - Added on July 27, 2017 at 12:02 PM by Anna Osipovich