This mouse line was made by gene targeting and designed to express an HA-tagged version of the transcription factor Neurog3 (Ngn3). The Lox71 and Lox2272 sites were inserted flanking the Ngn3 coding sequence. Within the flanked region an HA-tag was placed at the N-terminus of the Ngn3 protein. This allele can be used to study chromatin and protein-protein interactions of Ngn3. Additionally, the Lox71 and Lox2272 sites allow for manipulations of the flanked region by Recombinase-Mediated Cassete Exchange (RMCE) in ES cells.

 


Record History
Added on July 7, 2015 at 10:27 AM by Magnuson, Mark
Modified on October 31, 2019 at 2:38 PM by Skelton, Jennifer
Shared with (contributions)
VCMR: Vanderbilt Cryopreserved Mouse Repository
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Mouse Information

Common Name Neurog3(HA.LCA)
VCMR ID XG
Date Cryopreserved 2017-05-23
Method of Cryopreservation Sperm
Trial IVF % Fertilization 93.15%

Genetic Alteration

Mutation #1: Targeted Mutagenesis
Allele Name: neurogenin 3; targeted mutation, Guoqiang Gu
Symbol: Neurog3(HA.LCA)Ggu
Zygosity at cryopreservation Homozygote
PCR Genotyping Protocol Neurog3.HA_PCR_genotyping_protocol.docx
Citations Not provided

Background Strain Information

Strain Type Congenic Strain
Chimera/Founder Genetic Background 129S6/SvEvTac
Cryopreservation Strain Background (VCMR) CD-1
Viability and Fertility Data

Strain background:  90% CD-1 at cryopreservation.

Homozygous viable with reduced fertility.

Heterozygous viable and fertile.

Additional Information

Cryopreserved in homozygous state Neurog3{(HA/HA)}.

The targeting vector contains a 5.5 kb 5’ arm and a 3.5 kb 3’arm.  Lox71 and Lox2272 sites were inserted flanking the Ngn3 gene, where Ngn3 cDNA was replaced with that encoding for an HA-tagged Ngn3 protein.  The vector also contains a FRT-flanked Pgk-Puro-deltaTK cassette for positive selection by puromycin (targeting events) and negative selection by ganciclovir (RMCE events).

Attachments

Document Magnuson_Gu_Ngn3.HA.LCA_targeting.pptx - Added on February 15, 2018 at 10:47 AM by Jennifer Skelton


Document Ngn3_HA_staining.png - Added on July 27, 2017 at 12:02 PM by Anna Osipovich