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Rat Metabolic Physiology Sub-Core Procedures

List of procedures performed by the Rat Metabolic Physiology Sub-Core

Category
Service
Record History
Added on July 31, 2015 at 2:01 PM by Escue, Alisa
Modified on September 29, 2015 at 11:44 AM by Lindner, Jill
Shared with (contributions)
MPSR: Rat Metabolic Physiology Sub-Core Services Master

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Insulin Clamp

  • Peripheral infusion of 3-3H-Glucose and Insulin
  • Peripheral infusion of Glucose to maintain desired concentration
  • Blood sampled via arterial catheter
  • 14C-2-Deoxyglucose optional  (measure muscle glucose uptake)  

Pancreatic Clamp

  • Peripheral infusion of 3-3H-Glucose and somatostation (inhibit endogenous production of insulin & glucagon
  • Intraportal infusion of insulin and glucagon at set concentrations
  • Peripheral infusion of glucose to maintain desired concentration
  • Blood sampled via arterial &/or portal vein catheter
  • 14C optional

Hyperglycemic Clamp

  • Peripheral infusion of glucose to maintain desired concentration, typicall 200 mg/dl measured by glucometer
  • Blood sampled via arterial catheter

Tolerance Test

  • Glucose or Insulin
  • Oral, Intravenous, or Intraperitoneal
  • Blood sampled via arterial catheter

Pharmacokinetic Study (PK)

  • Administration of substrate(s) by single infusion or bolus via venous catheter, oral gavage, IP injection, or SC injection
  • Blood sampled from arterial catheter 

Blood Pressure Measurement

  • Requires access to cannulated artery, typically carotid artery
  • Arterial catheter conneced to an external pressure transducer
  • Data recorded using BioPac software

 Dosing Techniques   

  • Oral Gavage
  • Subcutaneous injection
  • Intraperitoneal injection
  • Intravenous injection  (catheter or tail vein)

Sampling Techniques 

  • Tail vein under anesthesia
  • Tail clip
  • Surgical catheters
  • Cardiac puncture
  • Cisterna magna under anesthesia