Sample volume requirements and range of detection


Category
Page
Record History
Added on July 30, 2015 at 1:21 PM by Edgerton, Dale
Modified on September 8, 2017 at 2:32 PM by Edgerton, Dale
Shared with (contributions)
HAASC: Hormone Assay Core Services Master

Assay

Requirements for Human/Canine

Requirements for Mouse/Rat

ACTH

(RIA)

400 µl plasma for duplicate analysis. 5-day double antibody assay. Detection range: 5-100 pg/ml.

55 µl plasma for duplicate analysis. 5-day double antibody assay. Detection range: 5-100 pg/ml.

Aldosterone

(RIA)

225 µl plasma for duplicate analysis. 2-day double antibody assay. Detection range: 5-100 pg/ml.

60 µl plasma for duplicate analysis. 2-day double antibody assay. Detection range: 5-100 pg/ml.

Amino Acids - Full and Select Profiles (e.g. gluconeogenic)

(HPLC)

100 µl plasma. Detection range: >1 µmol/l.

50 µl plasma. Detection range: >1 µmol/l.

Catecholamines Epinephrine and Norepinephrine

(HPLC)

250 µl plasma preserved in EGTA-glutathione. 1-day assay. Detection range: 50-2000 pg/ml.

50 µl plasma preserved in EGTA-glutathione 1-day assay. Detection range: 50-2000 pg/ml.

Corticosterone

(RIA)

NA

15 µl plasma for duplicate analysis. 1-day double antibody assay. Detection range: 25-1000 ng/ml.

Cortisol - Plasma

(RIA)

100 µl plasma for duplicate analysis. 1-day coated tube assay. Detection range: 0.5-50 µg/dl.

NA

Cortisol - Salivary

(RIA)

400 µl saliva for duplicate analysis. 5-day double antibody assay. Detection range: 0.5-20 ng/ml.

NA

C-peptide

(RIA)

400 µl plasma for duplicate analysis in EDTA/Trasylol. 2-day double antibody assay. Detection range: 0.1-10 ng/ml.

Available as a Luminex assay

Creatinine

(HPLC)

NA

Minimum of 30 µl plasma.

Ghrelin - Active

(RIA)

400 µl plasma for duplicate analysis in HCL/PMSF. 2-day double antibody assay. Detection range: 8-250 pg/ml.

30-60 µl plasma for duplicate analysis in HCL/PMSF. 5-day double antibody assay. Detection range: 5-100 pg/ml.

Ghrelin - Total

(RIA)

400 µl plasma for duplicate analysis. 2-day double antibody assay. Detection range: 150-5000 pg/ml.

30-60 µl plasma for duplicate analysis. 5-day double antibody assay. Detection range: 100-1000 pg/ml.

Glucagon (ELISA)

20 µl plasma for duplicate analysis. This assay has improved sensitivity and specificity and requires a smaller sample volume. Detection range: 2-180 pmol/L.

20 µl plasma for duplicate analysis. This assay has improved sensitivity and specificity and requires a smaller sample volume. Detection range: 2-180 pmol/L.

Glucagon

(RIA)

400 µl of plasma for duplicate analysis preserved in EDTA/Trasylol. 3-day double antibody assay. Detection range: 30-400 pg/ml.

60 µl plasma for samples with basal glucagon concentrations (~50 pg/ml). 30 µl plasma for concentrations >100 pg/ml. Duplicate analysis in plasma preserved in EDTA/Trasylol. 5-day double antibody assay. Detection range: 10-200 pg/ml.

Glucose

(Enzymatic)

100 µl of plasma for duplicate analysis. Detection range: 20-900 mg/dl.

40 µl of plasma for duplicate analysis. Detection range: 20-900 mg/dl.

Growth Hormone

(RIA)

400 µl plasma for duplicate analysis.1-day assay. Detection range: 0.5-50 ng/ml.

Available as a Luminex assay

Insulin

(RIA)

400 µl plasma for duplicate analysis. 2-day double antibody assay. Detection range: 1-150 µU/ml.

30 µl plasma for duplicate analysis. 5-day double antibody assay w/dilution procedure. Detection range: 0.01- 0.5 or 0.1-10 ng/ml. Interspecies or human specific assays are available.

Leptin

(RIA)

400 µl plasma for duplicate analysis. 2-day double antibody assay. Detection range: 0.5-100 ng/ml. FOR HUMAN ONLY - No canine assay available.

Available as a Luminex assay

Luminex Assays

(Fluorescence)

All analytes within a panel can be measured simultaneously from a single sample. Contact us for help with your kit selection.

Most Frequently Used:

Human Adipokine Panel 1 [20 µl - Adiponectin (total), Resistin, PAI-1 (total), Lipocalin-2/NGAL, Adipsin].

Human Cytokine/Chemokine Panel 1 [60 µl - EGF, Eotaxin/CCL11, FGF-2, Flt-3 Ligand, Fractalkine/CX3CL1, G-CSF, GM-CSF, GRO, IFNa2, IFN-y, IL-1a, IL-1b, IL-1ra, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8CXCL8, IL-9, IL-10, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17A, IP-10, MCP-1/CCL2/CXDL10, MCP-3/CCL7, MDC/CCL22, MIP-1a/CCL3, MIP-1b/CCL4, PDGF-AA, PDGF-AB/BB, RANTES/CCL5, sCD40L, TGFa, TNFa, TNFb/LTA, VEGF].

Human Metabolic Hormone [60 µl - Active Amylin*, Total Amylin*, C-Peptide, Ghrelin (active)*, GIP (total), GLP-1(active)*, GLP-1 (total)*, Glucagon*, IL-6, Insulin, Leptin, MCP-1/CCL2, Pancreatic Polypeptide, PYY (total), TNFa - *Needs preservative].

Human IGF [60 µl - IGF-1, IGF-2].

Human Th17 [60 µl - GM-CSF, INFy, IL-1b, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12(p70), IL-13, IL-15, IL-17A, IL-17E/IL-25, IL-17F, IL-21, IL-22, IL-23, IL-27, IL-28A, IL-31, IL-33, MIP-3a/CCL20, TNFa, TNFb/LTA].

Most Frequently Used:

Mouse Single Plex Adiponectin [7 µl - Adiponectin (total)].

Mouse Cytokine/Chemokine Panel 1 [5-60 µl depending on dilution - Eotaxin/CCL11, G-CSF, GM-CSF, IFN-y, IL-1a, IL-1b, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17A, IP-10/CXCL10, KC/CXCL1, LIF, LIX, MCP-1/CCL2, M-CSF, MIG/CXCL9, MIP-1a/CCL3, MIP-1b/CCL4, MIP-2/CCL8, RANTES/CCL5, TNFa, VEGF].

Mouse Pituitary [25 µl - ACTH, BDNF, FSH, GH, LH, Prolactin, TSH].

 Mouse/Rat IGF-1 [10 µl - IGF-1].

Mouse Th17 [60 µl - GM-CSF, INFy, IL-1b, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12(p70), IL-13, IL-15, IL-17A, IL-17E/IL-25, IL-17F, IL-21, IL-22, IL-23, IL-27, IL-28A, IL-28B, IL-31, IL-33, MIP-3a/CCL20, TNFa, TNFb/LTA].

Progesterone

(RIA)

400 µl plasma for duplicate analysis. 1-day double antibody assay. Detection range: 0.2-50 ng/ml.

35 µl plasma for duplicate analysis. 2-day double antibody assay. Detection range: 10-5000 pg/ml.

Purine Nucleotides

(HPLC)

In situ collected and weighed, 100 mg of liver (liquid nitrogen), add 1 ml cold 0.4M perchloric acid with 0.5M EGTA, homogenize, sit 10 min on ice, spin ~3200g@4C for 10 min, decant supernatant, add 8 drops 0.5M K2CO3, mix, sit 5 min on ice, spin ~3200g@4C for 5 min, decant, save supernatant, freeze / analyze.

In situ collected and weighed, 100 mg of liver (liquid nitrogen), add 1 ml cold 0.4M perchloric acid with 0.5M EGTA, homogenize, sit 10 min on ice, spin ~3200g@4C for 10 min, decant supernatant, add 8 drops 0.5M K2CO3, mix, sit 5 min on ice, spin ~3200g@4C for 5 min, decant, save supernatant, freeze / analyze.

Testosterone

(RIA)

400 µl plasma for duplicate analysis. 1-day double antibody assay. Detection range: 5-1000 pg/ml.

25 µl (male) or 35 µl (female) plasma for duplicate analysis. 2-day double antibody assay. Detection range: 5-1000 pg/ml.

T3

(RIA)

NA

30-60 µl plasma for duplicate analysis. 5-day double antibody assay. Detection range: 0.05-5 ng/ml.

T4

(RIA)

NA

30 µl plasma for duplicate analysis. 5-day double antibody assay. Detection range: 0.5-20 ng/ml.

 

 Click here for more information