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Lipid Core Plasma Lipid Profiles Method

Method for plasma lipid profiles

Category
Protocol
Record History
Added on July 29, 2015 at 2:26 PM by Harris, Carla
Modified on February 12, 2018 at 8:03 AM by Harris, Carla
Shared with (contributions)
HAASC: Lipid Core Master

Plasma Lipid Profiles

Total plasma cholesterol and triglyceride are measured by standard enzymatic assays.  HDL cholesterol is measured with the enzymatic method after precipitation of VLDL and LDL using polyethylene glycol reagent (PEG).  From these data LDL cholesterol is calculated using the Friedewald equation, as long as triglyceride levels are below 400 mg/dl. Investigators may request a total plasma lipid profile or specific plasma lipid measurements.

Plasma Enzymatic Free Fatty Acids (FFA) 

Plasma FFAs are analyzed with a commercially available enzymatic kit from Wako Life Sciences.

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