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Sample preparation, requirements, and shipping instructions for lipid assays


Category
Protocol
Record History
Added on July 29, 2015 at 12:04 PM by Harris, Carla
Modified on October 19, 2017 at 3:27 PM by Harris, Carla
Shared with (contributions)
HAASC: Lipid Core Master

Sample Preparation and Volumes Required for Lipid Assays

  • Tissue/cell samples should be frozen in liquid nitrogen immediately after collection.
  • Serum or EDTA plasma should be removed from the red cells as soon as possible after collection and then frozen.
  • Please label all tubes clearly and legibly.
  • All samples should be stored at -70°C until shipped.
  • Heparin is not acceptable as an anticoagulant for lipid profiles.
  • Samples with gross hemolysis or lipemia may yield false results.
  • Complete the order submission form online, make a copy, and place it in the container with your samples enclosed in a plastic bag.

Assay

Requirements for Human/Canine

Requirements for Mouse/Rat

Plasma/Serum Cholesterol

15 µl  plasma or serum

15 µl  plasma or serum

Plasma/Serum Triglycerides

15 µl plasma or serum

15 µl plasma or serum

Plasma/Serum HDL Cholesterol

30 µl  plasma or serum

30 µl  plasma or serum

Plasma/Serum enzymatic FFA

10 µl plasma or serum

10 µl plasma or serum

Plasma/Serum FFA by G.C. 

550 ul serum or EDTA plasma

150 ul--mouse  550 ul--rat

 

 

 

Tissue/Cell Cholesterol

~100 mg tissue

~100 mg tissue

Tissue/Cell Diglyceride

~100 mg tissue

~100 mg tissue

Tissue/Cell Free Fatty Acids

 ~100 mg tissue

 ~100 mg tissue

Tissue/Cell Phospholipid

~100 mg tissue

~100 mg tissue

Tissue/Cell Triglycerides

~100 mg tissue

~100 mg tissue

Plasma FPLC Lipoprotein Separation

150 µl plasma

150 µl plasma

 

 

Outside investigators should ship samples overnight on dry ice to:

Carla Harris

VUMC Lipid Core

1161 21st Avenue South

Room CC-3327 MCN

Nashville, TN 37232-2561

Please ship samples Monday through Thursday so that the Core receives them no later than Friday of any given week.

Internal investigators should deliver samples to MCN CC-3327.

For additional information concerning Lipid Profiles, please contact Larry Swift at 615-343-2646 or Carla Harris at 615-343-2645.

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