Lipid Core Sample Preparation, Sample Requirements, and Shipping Instructions

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Sample preparation, requirements, and shipping instructions for lipid assays

Category: Protocol
Record History: Added on July 29, 2015 at 12:04 PM by Harris, Carla
Modified on October 19, 2017 at 3:27 PM by Harris, Carla
Shared with (contributions): HAASC: Lipid Core

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Sample Preparation and Volumes Required for Lipid Assays

Tissue/cell samples should be frozen in liquid nitrogen immediately after collection.
Serum or EDTA plasma should be removed from the red cells as soon as possible after collection and then frozen.
Please label all tubes clearly and legibly.
All samples should be stored at -70°C until shipped.
Heparin is not acceptable as an anticoagulant for lipid profiles.
Samples with gross hemolysis or lipemia may yield false results.
Complete the order submission form online, make a copy, and place it in the container with your samples enclosed in a plastic bag.

Assay	Requirements for Human/Canine	Requirements for Mouse/Rat
Plasma/Serum Cholesterol	15 µl plasma or serum	15 µl plasma or serum
Plasma/Serum Triglycerides	15 µl plasma or serum	15 µl plasma or serum
Plasma/Serum HDL Cholesterol	30 µl plasma or serum	30 µl plasma or serum
Plasma/Serum enzymatic FFA	10 µl plasma or serum	10 µl plasma or serum
Plasma/Serum FFA by G.C.	550 ul serum or EDTA plasma	150 ul--mouse 550 ul--rat

Tissue/Cell Cholesterol	~100 mg tissue	~100 mg tissue
Tissue/Cell Diglyceride	~100 mg tissue	~100 mg tissue
Tissue/Cell Free Fatty Acids	~100 mg tissue	~100 mg tissue
Tissue/Cell Phospholipid	~100 mg tissue	~100 mg tissue
Tissue/Cell Triglycerides	~100 mg tissue	~100 mg tissue
Plasma FPLC Lipoprotein Separation	150 µl plasma	150 µl plasma

Outside investigators should ship samples overnight on dry ice to:

Carla Harris

VUMC Lipid Core

1161 21st Avenue South

Room CC-3327 MCN

Nashville, TN 37232-2561

Please ship samples Monday through Thursday so that the Core receives them no later than Friday of any given week.

Internal investigators should deliver samples to MCN CC-3327.

For additional information concerning Lipid Profiles, please contact Larry Swift at 615-343-2646 or Carla Harris at 615-343-2645.