Sample Preparation and Volumes Required for Lipid Assays
Assay |
Requirements for Human/Canine |
Requirements for Mouse/Rat |
Plasma/Serum Cholesterol |
15 µl plasma or serum |
15 µl plasma or serum |
Plasma/Serum Triglycerides |
15 µl plasma or serum |
15 µl plasma or serum |
Plasma/Serum HDL Cholesterol |
30 µl plasma or serum |
30 µl plasma or serum |
Plasma/Serum enzymatic FFA |
10 µl plasma or serum |
10 µl plasma or serum |
Plasma/Serum FFA by G.C. |
550 ul serum or EDTA plasma |
150 ul--mouse 550 ul--rat |
|
|
|
Tissue/Cell Cholesterol |
~100 mg tissue |
~100 mg tissue |
Tissue/Cell Diglyceride |
~100 mg tissue |
~100 mg tissue |
Tissue/Cell Free Fatty Acids |
~100 mg tissue |
~100 mg tissue |
Tissue/Cell Phospholipid |
~100 mg tissue |
~100 mg tissue |
Tissue/Cell Triglycerides |
~100 mg tissue |
~100 mg tissue |
Plasma FPLC Lipoprotein Separation |
150 µl plasma |
150 µl plasma |
Outside investigators should ship samples overnight on dry ice to:
Carla Harris
VUMC Lipid Core
1161 21st Avenue South
Room CC-3327 MCN
Nashville, TN 37232-2561
Please ship samples Monday through Thursday so that the Core receives them no later than Friday of any given week.
Internal investigators should deliver samples to MCN CC-3327.
For additional information concerning Lipid Profiles, please contact Larry Swift at 615-343-2646 or Carla Harris at 615-343-2645.