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Hormone Assay Core Services

Services provided by the Hormone Assay & Analytical Services Core

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Record History
Added on July 27, 2015 at 2:34 PM by Edgerton, Dale
Modified on March 29, 2022 at 9:57 AM by Edgerton, Dale
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Public: Shared Collection
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Radioimmunoassays (RIA)

  • ACTH, corticosterone, cortisol, c-peptide, ghrelin, glucagon, insulin, leptin, PYY, testosterone, T3 and T4

High Performance Liquid Chromatography (HPLC)

  • amino acids and catecholamines (epinephrine & norepinephrine)

Luminex multiplex assays

  • human and mouse adipokine, cytokine/chemokine, metabolic hormones, IGF, immunology/inflammation and pituitary assays

ELISA

  • glucagon

Enzymatic

  • glucose

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Resources

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Publications / Citations

  1. Insulin Delivery Into the Peripheral Circulation: A Key Contributor to Hypoglycemia in Type 1 Diabetes. Gregory JM, Kraft G, Scott MF, Neal DW, Farmer B, Smith MS, Hastings JR, Allen EJ, Donahue EP, Rivera N, Winnick JJ, Edgerton DS, Nishimura E, Fledelius C, Brand CL, Cherrington AD (2015) Diabetes 64(10): 3439-51
    › Primary publication · 26085570 (PubMed) · PMC4587648 (PubMed Central) · Added on 7/15/2015
  2. Acute effects of hyperinsulinemia and hyperglycemia on vascular inflammatory biomarkers and endothelial function in overweight and obese humans. Perkins JM, Joy NG, Tate DB, Davis SN (2015) Am J Physiol Endocrinol Metab 309(2): E168-76
    › Primary publication · 26015434 (PubMed) · PMC4504937 (PubMed Central) · Added on 7/30/2015
  3. Tissue inflammation and nitric oxide-mediated alterations in cardiovascular function are major determinants of endotoxin-induced insulin resistance. House LM, Morris RT, Barnes TM, Lantier L, Cyphert TJ, McGuinness OP, Otero YF (2015) Cardiovasc Diabetol : 56
    › Primary publication · 25986700 (PubMed) · PMC4484635 (PubMed Central) · Added on 7/30/2015
  4. Effects of Antecedent GABA A Receptor Activation on Counterregulatory Responses to Exercise in Healthy Man. Hedrington MS, Tate DB, Younk LM, Davis SN (2015) Diabetes 64(9): 3253-61
    › Primary publication · 25901095 (PubMed) · PMC4542446 (PubMed Central) · Added on 7/30/2015
  5. Chronic overeating impairs hepatic glucose uptake and disposition. Coate KC, Kraft G, Shiota M, Smith MS, Farmer B, Neal DW, Williams P, Cherrington AD, Moore MC (2015) Am J Physiol Endocrinol Metab 308(10): E860-7
    › Primary publication · 25783892 (PubMed) · PMC4587587 (PubMed Central) · Added on 7/24/2015
  6. Counterregulatory responses to hypoglycemia differ between glimepiride and glyburide in non diabetic individuals. Joy NG, Tate DB, Davis SN (2015) Metabolism 64(6): 729-37
    › Primary publication · 25765720 (PubMed) · PMC4408247 (PubMed Central) · Added on 7/30/2015
  7. Effects of Acute and Antecedent Hypoglycemia on Endothelial Function and Markers of Atherothrombotic Balance in Healthy Humans. Joy NG, Tate DB, Younk LM, Davis SN (2015) Diabetes 64(7): 2571-80
    › Primary publication · 25695946 (PubMed) · PMC4477350 (PubMed Central) · Added on 7/30/2015
  8. Human islet preparations distributed for research exhibit a variety of insulin-secretory profiles. Kayton NS, Poffenberger G, Henske J, Dai C, Thompson C, Aramandla R, Shostak A, Nicholson W, Brissova M, Bush WS, Powers AC (2015) Am J Physiol Endocrinol Metab 308(7): E592-602
    › Primary publication · 25648831 (PubMed) · PMC4385877 (PubMed Central) · Added on 7/28/2015
  9. Interleukin-6 amplifies glucagon secretion: coordinated control via the brain and pancreas. Barnes TM, Otero YF, Elliott AD, Locke AD, Malabanan CM, Coldren AG, Brissova M, Piston DW, McGuinness OP (2014) Am J Physiol Endocrinol Metab 307(10): E896-905
    › Primary publication · 25205821 (PubMed) · PMC4233256 (PubMed Central) · Added on 10/7/2014
  10. Incorporation of therapeutically modified bacteria into gut microbiota inhibits obesity. Chen Z, Guo L, Zhang Y, Walzem RL, Pendergast JS, Printz RL, Morris LC, Matafonova E, Stien X, Kang L, Coulon D, McGuinness OP, Niswender KD, Davies SS (2014) J Clin Invest 124(8): 3391-406
    › Primary publication · 24960158 (PubMed) · PMC4109548 (PubMed Central) · Added on 7/21/2014
  11. Changes in glucose and fat metabolism in response to the administration of a hepato-preferential insulin analog. Edgerton DS, Moore MC, Winnick JJ, Scott M, Farmer B, Naver H, Jeppesen CB, Madsen P, Kjeldsen TB, Nishimura E, Brand CL, Cherrington AD (2014) Diabetes 63(11): 3946-54
    › Primary publication · 24947349 (PubMed) · PMC4392933 (PubMed Central) · Added on 1/20/2015
  12. Hepatic glucose uptake and disposition during short-term high-fat vs. high-fructose feeding. Coate KC, Kraft G, Moore MC, Smith MS, Ramnanan C, Irimia JM, Roach PJ, Farmer B, Neal DW, Williams P, Cherrington AD (2014) Am J Physiol Endocrinol Metab 307(2): E151-60
    › Primary publication · 24865981 (PubMed) · PMC4101635 (PubMed Central) · Added on 6/2/2014
  13. Liver but not adipose tissue is responsive to the pattern of enteral feeding. Otero YF, Lundblad TM, Ford EA, House LM, McGuinness OP (2014) Physiol Rep 2(2): e00250
    › Primary publication · 24744913 (PubMed) · PMC3966249 (PubMed Central) · Added on 7/21/2014
  14. Hypoglycemia-associated autonomic failure in healthy humans: comparison of two vs three periods of hypoglycemia on hypoglycemia-induced counterregulatory and symptom response 5 days later. Moheet A, Kumar A, Eberly LE, Kim J, Roberts R, Seaquist ER (2014) J Clin Endocrinol Metab 99(2): 664-70
    › Primary publication · 24423306 (PubMed) · PMC3913799 (PubMed Central) · Added on 7/30/2015
  15. Biobehavioral profiles of arousal and social motivation in autism spectrum disorders. Corbett BA, Swain DM, Newsom C, Wang L, Song Y, Edgerton D (2014) J Child Psychol Psychiatry 55(8): 924-34
    › Primary publication · 24329926 (PubMed) · PMC4055549 (PubMed Central) · Added on 3/10/2014
  16. Cholesteryl ester transfer protein protects against insulin resistance in obese female mice. Cappel DA, Palmisano BT, Emfinger CH, Martinez MN, McGuinness OP, Stafford JM (2013) Mol Metab 2(4): 457-67
    › Primary publication · 24327961 (PubMed) · PMC3854988 (PubMed Central) · Added on 2/27/2014
  17. Effect of the glucagon-like peptide-1 receptor agonist lixisenatide on postprandial hepatic glucose metabolism in the conscious dog. Moore MC, Werner U, Smith MS, Farmer TD, Cherrington AD (2013) Am J Physiol Endocrinol Metab 305(12): E1473-82
    › Primary publication · 24148347 (PubMed) · PMC3882379 (PubMed Central) · Added on 6/2/2014
  18. Novel PEGylated basal insulin LY2605541 has a preferential hepatic effect on glucose metabolism. Moore MC, Smith MS, Sinha VP, Beals JM, Michael MD, Jacober SJ, Cherrington AD (2014) Diabetes 63(2): 494-504
    › Primary publication · 24089512 (PubMed) · PMC5361402 (PubMed Central) · Added on 6/2/2014
  19. A liver Hif-2α-Irs2 pathway sensitizes hepatic insulin signaling and is modulated by Vegf inhibition. Wei K, Piecewicz SM, McGinnis LM, Taniguchi CM, Wiegand SJ, Anderson K, Chan CW, Mulligan KX, Kuo D, Yuan J, Vallon M, Morton L, Lefai E, Simon MC, Maher JJ, Mithieux G, Rajas F, Annes J, McGuinness OP, Thurston G, Giaccia AJ, Kuo CJ (2013) Nat Med 19(10): 1331-1337
    › Primary publication · 24037094 (PubMed) · PMC3795838 (PubMed Central) · Added on 7/21/2014
  20. A novel experimental strategy to assess the metabolic effects of selective activation of a G(q)-coupled receptor in hepatocytes in vivo. Li JH, Jain S, McMillin SM, Cui Y, Gautam D, Sakamoto W, Lu H, Jou W, McGuinness OP, Gavrilova O, Wess J (2013) Endocrinology 154(10): 3539-51
    › Primary publication · 23861369 (PubMed) · PMC3776870 (PubMed Central) · Added on 7/21/2014
  21. Effects of intraportal exenatide on hepatic glucose metabolism in the conscious dog. Edgerton DS, An Z, Johnson KM, Farmer T, Farmer B, Neal D, Cherrington AD (2013) Am J Physiol Endocrinol Metab 305(1): E132-9
    › Primary publication · 23673158 (PubMed) · PMC3725568 (PubMed Central) · Added on 7/21/2014
  22. G6PC2: a negative regulator of basal glucose-stimulated insulin secretion. Pound LD, Oeser JK, O'Brien TP, Wang Y, Faulman CJ, Dadi PK, Jacobson DA, Hutton JC, McGuinness OP, Shiota M, O'Brien RM (2013) Diabetes 62(5): 1547-56
    › Primary publication · 23274894 (PubMed) · PMC3636628 (PubMed Central) · Added on 7/21/2014
  23. Portal vein glucose entry triggers a coordinated cellular response that potentiates hepatic glucose uptake and storage in normal but not high-fat/high-fructose-fed dogs. Coate KC, Kraft G, Irimia JM, Smith MS, Farmer B, Neal DW, Roach PJ, Shiota M, Cherrington AD (2013) Diabetes 62(2): 392-400
    › Primary publication · 23028137 (PubMed) · PMC3554368 (PubMed Central) · Added on 12/5/2013
  24. Portal glucose delivery stimulates muscle but not liver protein metabolism. Kraft G, Coate KC, Dardevet D, Farmer B, Donahue EP, Williams PE, Cherrington AD, Moore MC (2012) Am J Physiol Endocrinol Metab 303(10): E1202-11
    › Primary publication · 23011060 (PubMed) · PMC3774325 (PubMed Central) · Added on 12/5/2013
  25. Estrogen treatment after ovariectomy protects against fatty liver and may improve pathway-selective insulin resistance. Zhu L, Brown WC, Cai Q, Krust A, Chambon P, McGuinness OP, Stafford JM (2013) Diabetes 62(2): 424-34
    › Primary publication · 22966069 (PubMed) · PMC3554377 (PubMed Central) · Added on 12/10/2013
  26. Liver glycogen loading dampens glycogen synthesis seen in response to either hyperinsulinemia or intraportal glucose infusion. Winnick JJ, An Z, Kraft G, Ramnanan CJ, Irimia JM, Smith M, Lautz M, Roach PJ, Cherrington AD (2013) Diabetes 62(1): 96-101
    › Primary publication · 22923473 (PubMed) · PMC3526057 (PubMed Central) · Added on 12/5/2013
  27. Neuron-specific deletion of peroxisome proliferator-activated receptor delta (PPARδ) in mice leads to increased susceptibility to diet-induced obesity. Kocalis HE, Turney MK, Printz RL, Laryea GN, Muglia LJ, Davies SS, Stanwood GD, McGuinness OP, Niswender KD (2012) PLoS One 7(8): e42981
    › Primary publication · 22916190 (PubMed) · PMC3423438 (PubMed Central) · Added on 12/10/2013
  28. The physiological effects of deleting the mouse SLC30A8 gene encoding zinc transporter-8 are influenced by gender and genetic background. Pound LD, Sarkar SA, Ustione A, Dadi PK, Shadoan MK, Lee CE, Walters JA, Shiota M, McGuinness OP, Jacobson DA, Piston DW, Hutton JC, Powell DR, O'Brien RM (2012) PLoS One 7(7): e40972
    › Primary publication · 22829903 (PubMed) · PMC3400647 (PubMed Central) · Added on 12/10/2013
  29. A cyclic guanosine monophosphate-dependent pathway can regulate net hepatic glucose uptake in vivo. An Z, Winnick JJ, Moore MC, Farmer B, Smith M, Irimia JM, Roach PJ, Cherrington AD (2012) Diabetes 61(10): 2433-41
    › Primary publication · 22688328 (PubMed) · PMC3447895 (PubMed Central) · Added on 6/2/2014
  30. Regulation of hepatic glucose uptake and storage in vivo. Moore MC, Coate KC, Winnick JJ, An Z, Cherrington AD (2012) Adv Nutr 3(3): 286-94
    › Primary publication · 22585902 (PubMed) · PMC3649460 (PubMed Central) · Added on 12/5/2013
  31. Characterization of the human SLC30A8 promoter and intronic enhancer. Pound LD, Sarkar SA, Cauchi S, Wang Y, Oeser JK, Lee CE, Froguel P, Hutton JC, O'Brien RM (2011) J Mol Endocrinol 47(3): 251-9
    › Primary publication · 21798992 (PubMed) · PMC3185167 (PubMed Central) · Added on 7/30/2015
  32. The pancreatic islet β-cell-enriched transcription factor Pdx-1 regulates Slc30a8 gene transcription through an intronic enhancer. Pound LD, Hang Y, Sarkar SA, Wang Y, Milam LA, Oeser JK, Printz RL, Lee CE, Stein R, Hutton JC, O'Brien RM (2011) Biochem J 433(1): 95-105
    › Primary publication · 20942803 (PubMed) · PMC4130494 (PubMed Central) · Added on 3/7/2014
  33. Continuous low-dose fructose infusion does not reverse glucagon-mediated decrease in hepatic glucose utilization. Johnson PM, Chen SS, Santomango TS, Williams PE, Lacy DB, McGuinness OP (2011) Metabolism 60(6): 867-73
    › Primary publication · 20940071 (PubMed) · PMC3736817 (PubMed Central) · Added on 12/10/2013
  34. Deletion of the mouse Slc30a8 gene encoding zinc transporter-8 results in impaired insulin secretion. Pound LD, Sarkar SA, Benninger RK, Wang Y, Suwanichkul A, Shadoan MK, Printz RL, Oeser JK, Lee CE, Piston DW, McGuinness OP, Hutton JC, Powell DR, O'Brien RM (2009) Biochem J 421(3): 371-6
    › Primary publication · 19450229 (PubMed) · PMC2929527 (PubMed Central) · Added on 12/10/2013
  35. Insulin and epidermal growth factor suppress basal glucose-6-phosphatase catalytic subunit gene transcription through overlapping but distinct mechanisms. Onuma H, Oeser JK, Nelson BA, Wang Y, Flemming BP, Scheving LA, Russell WE, O'Brien RM (2009) Biochem J 417(2): 611-20
    › Primary publication · 18847435 (PubMed) · PMC2929524 (PubMed Central) · Added on 3/7/2014
  36. Foxa2 and MafA regulate islet-specific glucose-6-phosphatase catalytic subunit-related protein gene expression. Martin CC, Flemming BP, Wang Y, Oeser JK, O'Brien RM (2008) J Mol Endocrinol 41(5): 315-28
    › Primary publication · 18753309 (PubMed) · PMC2614309 (PubMed Central) · Added on 7/30/2015
  37. Deletion of the gene encoding the islet-specific glucose-6-phosphatase catalytic subunit-related protein autoantigen results in a mild metabolic phenotype. Wang Y, Martin CC, Oeser JK, Sarkar S, McGuinness OP, Hutton JC, O'Brien RM (2007) Diabetologia 50(4): 774-8
    › Primary publication · 17265032 (PubMed) · Added on 12/10/2013
  38. Differential regulation of islet-specific glucose-6-phosphatase catalytic subunit-related protein gene transcription by Pax-6 and Pdx-1. Martin CC, Oeser JK, O'Brien RM (2004) J Biol Chem 279(33): 34277-89
    › Primary publication · 15180990 (PubMed) · Added on 7/30/2015
  39. Identification and characterization of a cDNA and the gene encoding the mouse ubiquitously expressed glucose-6-phosphatase catalytic subunit-related protein. Boustead JN, Martin CC, Oeser JK, Svitek CA, Hunter SI, Hutton JC, O'Brien RM (2004) J Mol Endocrinol 32(1): 33-53
    › Primary publication · 14765991 (PubMed) · Added on 7/10/2013
  40. Identification and characterization of a human cDNA and gene encoding a ubiquitously expressed glucose-6-phosphatase catalytic subunit-related protein. Martin CC, Oeser JK, Svitek CA, Hunter SI, Hutton JC, O'Brien RM (2002) J Mol Endocrinol 29(2): 205-22
    › Primary publication · 12370122 (PubMed) · Added on 7/10/2013

MeSH Terms

Acetaminophen Acyltransferases Adiposity Administration, Intravenous Adolescent Adult Aged Alleles Alprazolam Amino Acid Motifs Amino Acids Amino Acid Sequence Amino Acid Transport System ASC AMP-Activated Protein Kinases Animals Antidiuretic Hormone Receptor Antagonists Apolipoprotein B-100 Arabidopsis Proteins Arousal Arterial Pressure Autoantigens Autonomic Nervous System Base Sequence Basic Helix-Loop-Helix Transcription Factors Bicycling Binding Sites Biocatalysis Biological Transport Biomarkers Blood Glucose Blood Pressure Body Composition Body Weight Brain C-Peptide Calcium Signaling Cardiac Output Catalysis Catalytic Domain Cation Transport Proteins Cell Line Cell Line, Tumor Cell Nucleus Cells, Cultured Chemokine CCL2 Child Child Development Disorders, Pervasive Chloramphenicol O-Acetyltransferase Chlorocebus aethiops Chromatin Chromosomes Chromosomes, Human, Pair 17 Chronic Disease Cloning, Molecular Consciousness Conserved Sequence COS Cells Cricetinae Cyclic AMP Cyclic GMP Diabetes Mellitus Diabetes Mellitus, Experimental Diabetes Mellitus, Type 1 Diabetes Mellitus, Type 2 Diet, Fat-Restricted Diet, High-Fat Dietary Carbohydrates Dietary Fats Digestive System Disease Models, Animal DNA DNA, Complementary DNA-Binding Proteins DNA Mutational Analysis Dogs Dose-Response Relationship, Drug Eating Echocardiography Electrophoresis, Polyacrylamide Gel Endothelium, Vascular Endothelium-Dependent Relaxing Factors Energy Metabolism Enhancer Elements, Genetic Enteral Nutrition Enzyme Activators Epidermal Growth Factor Epinephrine Escherichia coli Estradiol Estrogen Receptor alpha Estrogens Exenatide Exercise Eye Proteins Fasting Fatty Liver Female Fibrinolysis Fructose G-Protein-Coupled Receptor Kinases GABA-A Receptor Agonists Gastric Emptying Gene Deletion Gene Expression Gene Expression Regulation Gene Expression Regulation, Enzymologic Genes, Reporter Glucagon Glucagon-Like Peptide-1 Receptor Glucagon-Secreting Cells Glucokinase Gluconeogenesis Glucose Glucose-6-Phosphatase Glucose-6-Phosphate Glucose Clamp Technique Glucose Intolerance Glucose Tolerance Test Glyburide Glycerol Glycogen Glycogenolysis Glycogen Phosphorylase Glycogen Synthase Glycolysis GTP-Binding Protein alpha Subunits, Gq-G11 Heart HeLa Cells Hemodynamics Hepatocyte Nuclear Factor 1 Hepatocyte Nuclear Factor 3-alpha Hepatocyte Nuclear Factor 3-beta Hepatocytes Heterozygote Homeodomain Proteins Hormones Human Growth Hormone Humans Hydrocortisone Hyperglycemia Hyperinsulinism Hyperphagia Hypoglycemia Hypoglycemic Agents Inflammation Inflammation Mediators Infusions, Intravenous Insulin Insulin-Secreting Cells Insulin Lispro Insulin Receptor Substrate Proteins Insulin Resistance Insulins Insulin Secretion Interleukin-6 Introns Islets of Langerhans Kinetics Lactic Acid Large Neutral Amino Acid-Transporter 1 Leptin Lipid Metabolism Lipids Lipolysis Lipopolysaccharides Liver Liver Glycogen Luciferases Maf Transcription Factors, Large Male Methylation Mice Mice, Congenic Mice, Inbred C57BL Mice, Knockout Mice, Obese Mice, Transgenic Microbiota Microspheres Middle Aged Models, Animal Molecular Sequence Data Motivation Muscle, Skeletal Muscle Cells Muscles Mutagenesis, Site-Directed Mutation Nitric Oxide Nitric Oxide Synthase Type II Norepinephrine Obesity Oligonucleotides Organ Size Ovariectomy Overweight Paired Box Transcription Factors Pancreas Pancreatectomy Pancreatic Polypeptide Parenteral Nutrition, Total PAX6 Transcription Factor Peer Group Peptides Phosphatidylethanolamines Phosphofructokinase-1 Phosphorylation Pituitary-Adrenal System Plasmids Platelet Activation Play and Playthings Polyethylene Glycols Polymerase Chain Reaction Portal Vein Postprandial Period PPAR delta Precipitin Tests Promoter Regions, Genetic Protein Binding Protein Biosynthesis Protein Engineering Protein Interaction Domains and Motifs Proteins Protein Structure, Tertiary Protein Subunits Pure Autonomic Failure Rats Receptor, Muscarinic M3 Receptors, GABA-A Receptors, Glucagon Receptors, Vasopressin Recombinant Fusion Proteins Recombinant Proteins Regional Blood Flow Repressor Proteins Research Ribosomal Protein S6 Kinases, 70-kDa RNA, Messenger Saliva Salts Sequence Alignment Sequence Deletion Serpin E2 Sex Characteristics Sex Factors Signal Transduction Single-Blind Method Social Behavior Somatostatin Specific Pathogen-Free Organisms Specimen Handling Stress, Physiological Stress, Psychological Subcellular Fractions Sulfonylurea Compounds Surveys and Questionnaires Sympathomimetics Thrombosis Tissue and Organ Procurement Tissue Distribution Tissue Donors Trans-Activators Transcription, Genetic Transcriptional Activation Transcription Factors Transfection Triglycerides Tumor Necrosis Factor-alpha Vascular Endothelial Growth Factor A Vasculitis Venoms Wechsler Scales Weight Gain Young Adult Zinc Zinc Transporter 8