This is an unpublished mouse strain in which the first exon of Pdx1 was replaced with coding sequences for Cre recombinase by gene targeting.  The mouse was developed by Mark Magnuson. 

Keywords: Cre Pdx1 Mgn

Record History
Added on July 7, 2015 at 10:28 AM by Magnuson, Mark
Modified on December 6, 2018 at 9:34 AM by Skelton, Jennifer
Shared with (contributions)
VCMR: Vanderbilt Cryopreserved Mouse Repository
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Mouse Information

Common Name Pdx1Cre
VCMR ID OV
Date Cryopreserved 2013-01-28
Method of Cryopreservation Sperm
Trial IVF % Fertilization 97.30%

Genetic Alteration

Mutation #1: RMCE Targeted Mutagenesis
Allele Name: pancreatic and duodenal homeobox 1; cre
Symbol: Pdx1cre
Zygosity at cryopreservation Heterozygote
PCR Genotyping Protocol Pdx1.Cre_PCR_genotyping_protocol.docx
Citations Not provided

Background Strain Information

Strain Type Congenic Strain
Chimera/Founder Genetic Background 129S6/SvEvTac
Cryopreservation Strain Background (VCMR) C57BL/6J
Viability and Fertility Data

Strain Background:  93.75% C57Bl6/J at cryopreservation

Homozgyous lethal at P0.
Heterozygous viable and fertile.

Additional Information

An 8.62 kb region of this gene has been replaced by tandemly oriented Lox66 and Lox2272 sites flanking positive (puromycin) and negative (HSV-TK) selectable markers.

The Pdx1.Cre exchange vector was made by cloning Cre coding sequences immediately following the translation initiation site in the Pdx1 gene.  The basal Pdx1 exchange vector contains all of the 8,610 bp of DNA from Pdx1 gene (promoter and exon 1 that is absent in the Pdx1LCA allele, Lox66 and Lox2272 sites, and a flrted (flanked by FRT) hygromycin selection cassette.

Attachment

Document pdx1crehygro.png - Added on July 7, 2015 at 10:28 AM by Mark Magnuson

The Pdx1.cre vector was exchanged into Pdx1LCA mESCs utilizing Recombinase Mediated Cassette Exchange (RMCE) and mice were generated from the correctly exchanged mESCs.  These animals were subsequently mated with Flpe mice in order to remove the HygroR cassette.